[CAS NO. 150849-52-8] WST-1

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PRODUCTS SPECIFICATIONS [150849-52-8]

Catalog

HY-136976

Brand

MCE

CAS

150849-52-8

DESCRIPTION [150849-52-8]

Overview

MDL	-
Molecular Weight	651.34
Molecular Formula	C19H11IN5NaO8S2
SMILES	O=[S]([O-])(C1=C(C=CC([S](=O)(O[Na])=O)=C1)C2=NN(C(C=C3)=CC=C3I)[N+](C(C=C4)=CC=C4[N+]([O-])=O)=N2)=O

For research use only. We do not sell to patients.

1 Publications Citing Use of MCE

[1]Biomaterials. 2022 Feb;281:121341.

Summary

WST-1 is a Tetrazolium dye, widely used in the cell viability assays. WST-1 produces a water-soluble formazan product (WST-1 formazan) that occurs at the cell surface in NADPH dependent manner in viable cells ^[1] ^[2] .

In Vitro

WST-1 is used for a wide range of assays that include cell viability, proliferation, plasma membrane electron transport system, superoxide generation, superoxide dismutase, and xanthine oxidase assay ^[1] .
A water-soluble formazan dye is produced from the reduction of WST-1 tetrazolium, of which optical density at 450 nm is measured to evaluate cell viability ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL ( 38.38 mM ; ultrasonic and warming and heat to 80°C)

H ₂ O : < 0.1 mg/mL (ultrasonic;warming;adjust pH to 9 with NaOH;heat to 80°C) (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5353 mL	7.6765 mL	15.3530 mL
5 mM	0.3071 mL	1.5353 mL	3.0706 mL
10 mM	0.1535 mL	0.7676 mL	1.5353 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.5 mg/mL (3.84 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (3.84 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

2H-Tetrazolium, 5-(2,4-disulfophenyl)-2-(4-iodophenyl)-3-(4-nitrophenyl)-, inner salt, sodium salt (1:1)

2H-Tetrazolium, 5-(2,4-disulfophenyl)-2-(4-iodophenyl)-3-(4-nitrophenyl)-, inner salt, sodium salt

5-(2,4-Disulfophenyl)-2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium inner salt sodium salt (1:1)

WST 1