[CAS NO. 1049741-55-0] CardiogenolChydrochloride
PRODUCTS SPECIFICATIONS [1049741-55-0]
DESCRIPTION [1049741-55-0]
Overview
| MDL | MFCD06798334 |
|---|---|
| Molecular Weight | 296.75 |
| Molecular Formula | C13H17ClN4O2 |
| SMILES | [H]Cl.COC1=CC=C(NC2=NC=CC(NCCO)=N2)C=C1 |
Summary
Cardiogenol C hydrochloride is a potent cell-permeable pyrimidine inducer which prompts the differentiation of ESCs into cardiomyocytes ( EC 50 =100 nM) [1] . Cardiogenol C hydrochloride also acts cardiomyogenic on already lineage-committed progenitor cell types with a limited degree of plasticity. Cardiogenol C hydrochloride is a useful cardiomyogenic agent and can be used as a tool to improve cardiac repair by cell transplantation therapy in animal models [2] .
IC50 & Target
EC50: 100 nM (differentiation of ESCs into cardiomyocytes) [1]
In Vitro
Cardiogenol C hydrochloride (1 μM; 7 days) has a cardiomyogenic effect on P19 cells, it significantly increases atrial natriuretic factor (ANF,
nppa
) in P19 cells when it compares to untreated control cells
[1]
.
Cardiogenol C hydrochloride (0.01-100 μM; 7 days) significantly increases ANF expression. In addition, another frequently used cardiac marker gene (NKX2-5) is also significantly increased by this small molecule in C2C12 cells
[2]
.
Cardiogenol C hydrochloride (0.001-100 μM; 7 days) increases cardiac Nav1.5 sodium channel protein expression as dose-dependent manner in C2C12 cells
[2]
.
Cardiogenol C hydrochloride (1 μM; 35 days) addition from day 0 significantly increases myocardial differentiation and results in a significantly increased percentage of CBs with beating cardiomyocytes. This small moleculepromotes the development of beating cardiomyocytes in cardiovascular progenitor cell-derived cardiac bodies
[2]
.
Cardiogenol C hydrochloride (0.01-100 μM; 7 days) does not effect cell growth even at 10 μM. In addition, Cardiogenol C either solves in water or DMSO generates a similar effect. The highest concentration, 100 μM has significant cellular toxicity on C2C12 cells
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
RT-PCR [2]
| Cell Line: | C2C12 cells |
| Concentration: | 0.01 μM; 0.1 μM; 1 μM; 3 μM; 10 μM; 30 μM; 100 μM |
| Incubation Time: | 7 days |
| Result: | Increased ANF and NKX2.5 mRNA level as a dose-dependent manner. |
Western Blot Analysis [2]
| Cell Line: | C2C12 cells |
| Concentration: | 0.001 μM; 0.01 μM; 0.1 μM; 1 μM; 10 μM |
| Incubation Time: | 7 days |
| Result: | Increased cardiac Nav1.5 sodium channel protein levels. |
Cell Proliferation Assay [2]
| Cell Line: | C2C12 cells |
| Concentration: | 0.01 μM; 0.1 μM; 1 μM |
| Incubation Time: | 7 days |
| Result: | Did not exert toxic effects on C2C12 cells at 0.01-10 μM treatment. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : ≥ 59 mg/mL ( 198.82 mM )
H 2 O : 2 mg/mL ( 6.74 mM ; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.3698 mL | 16.8492 mL | 33.6984 mL |
| 5 mM | 0.6740 mL | 3.3698 mL | 6.7397 mL |
| 10 mM | 0.3370 mL | 1.6849 mL | 3.3698 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 3.33 mg/mL (11.22 mM); Clear solution; Need ultrasonic
-
2.
-
3.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (8.42 mM); Clear solution
-
4.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (8.42 mM); Clear solution
References
- [1] Wu X, et al. Small molecules that induce cardiomyogenesis in embryonic stem cells.J Am Chem Soc. 2004 Feb 18;126(6):1590-1.
- [2] Mike AK, et al. Small molecule cardiogenol C upregulates cardiac markers and induces cardiac functional properties in lineage-committed progenitor cells. Cell Physiol Biochem. 2014;33(1):205-21.
