[CAS NO. 108321-05-7] Phosphoribosyl pyrophosphate pentasodium
PRODUCTS SPECIFICATIONS [108321-05-7]
DESCRIPTION [108321-05-7]
Overview
| MDL | MFCD00084655 |
|---|---|
| Molecular Weight | 499.98 |
| Molecular Formula | C5H8Na5O14P3 |
| SMILES | O[C@@H]1[C@H](O)[C@@H](COP(O[Na])(O[Na])=O)O[C@@H]1OP(OP(O[Na])(O[Na])=O)(O[Na])=O |
Summary
Phosphoribosyl pyrophosphate (PRPP) pentasodium is an important metabolite required in the biosynthesis of purine and pyrimidine nucleotides, the amino acids histidine and tryptophan, and the cofactors NAD and NADP [1] .
IC50 & Target
|
Human Endogenous Metabolite |
In Vitro
Phosphoribosyl diphosphate (PRPP) pentasodium is an important intermediate in cellular metabolism. PRPP is synthesized by PRPP synthase, as follows: ribose 5-phosphate + ATP → PRPP + AMP. PRPP is ubiquitously found in living organisms and is used in substitution reactions with the formation of glycosidic bonds. PRPP is utilized in the biosynthesis of purine and pyrimidine nucleotides, the amino acids histidine and tryptophan, the cofactors NAD and tetrahydromethanopterin, arabinosyl monophosphodecaprenol, and certain aminoglycoside antibiotics
[1]
.
Phosphoribosyl diphosphate (5-Phosphoribosyl-l-pyrophosphate) pentasodium, a substrate shared by adenine phosphoribosyltransferase and hypoxanthine-guanine phosphoribosyltransferase, accumulates in human erythrocytes lacking hypoxanthine-guanine phosphoribosyltransferase. 5-Phosphoribosyl-l-pyrophosphate added to purified adenine phosphoribosyltransferase stabilizes it against heat inactivation
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H 2 O : 50 mg/mL ( 100.00 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.0001 mL | 10.0004 mL | 20.0008 mL |
| 5 mM | 0.4000 mL | 2.0001 mL | 4.0002 mL |
| 10 mM | 0.2000 mL | 1.0000 mL | 2.0001 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 25 mg/mL (50.00 mM); Clear solution; Need ultrasonic and warming
References
- [1] Schneiter R, et al. The importance of the five phosphoribosyl-pyrophosphate synthetase (Prs) gene products of Saccharomyces cerevisiae in the maintenance of cell integrity and the subcellular localization of Prs1p. Microbiology (Reading). 2000;146 Pt 12:3
- [2] Greene ML, et al. Substrate stabilization: genetically controlled reciprocal relationship of two human enzymes. Science. 1970;167(3919):887-889.
