[CAS NO. 109664-02-0] Neocryptotanshinone
PRODUCTS SPECIFICATIONS [109664-02-0]
DESCRIPTION [109664-02-0]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 314.38 |
| Molecular Formula | C19H22O4 |
| SMILES | O=C1C([C@@H](C)CO)=C(O)C(C2=C1C=CC3=C2CCCC3(C)C)=O |
Summary
Neocryptotanshinone, a fatty diterpenoids from Salvia Miltiorrhiza , inhibits lipopolysaccharide-induced inflammation by suppression of NF-κB and iNOS signaling pathways [1] [2] .
IC50 & Target
Target: NF-κB, iNOS [1]
In Vitro
Neocryptotanshinone exhibits anti-inflammatory effect by suppression of NF-κB and iNOS signaling pathways
[1]
.
Neocryptotanshinone (10 μM and 20 μM, 24 h) inhibits LPS-induced iNOS protein expression in RAW264.7 cells
[1]
.
Neocryptotanshinone (20 μM, 24 h) shows no obvious cytotoxic effect towards murine RAW264.7 macrophages
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | RAW264.7 cells |
| Concentration: | 5, 10 and 20 μM |
| Incubation Time: | 24 hours |
| Result: | Inhibited LPS-induced cell viability in a dose-dependent manner. |
Western Blot Analysis [1]
| Cell Line: | RAW264.7 cells |
| Concentration: | 20 μM |
| Incubation Time: | 24 hours |
| Result: | Inhibited LPS-induced activation of NF-κB pathway and down-regulated LPS-induced expression of p-NF-κB p65, p-IκBα and p-IKKβ. |
In Vivo
Neocryptotanshinone causes reversals of decreased pain thresholds induced by MSU treatment after 30, 60, and 120 min S. miltiorrhiza Bunge extract treatment (contains single active components) [3] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | MSU-induced pain model in male ICR mice (weighing 20-25 g) [2] |
| Dosage: | 10, 25, 50 or 100 mg/kg |
| Administration: | Oral gavage; for 30, 60, 120 min |
| Result: | Inhibited inflammatory symptoms and nociceptive behaviors in a dose-dependent manner. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL ( 318.09 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.1809 mL | 15.9043 mL | 31.8086 mL |
| 5 mM | 0.6362 mL | 3.1809 mL | 6.3617 mL |
| 10 mM | 0.3181 mL | 1.5904 mL | 3.1809 mL |
References
- [1] Chuanhong Wu, et al. Neocryptotanshinone Inhibits Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages by Suppression of NF-κB and iNOS Signaling Pathways. Acta Pharm Sin B. 2015 Jul;5(4):323-9.
- [2] H C Lin, et al. Two New Fatty Diterpenoids From Salvia Miltiorrhiza. J Nat Prod. 2001 May;64(5):648-50.
- [3] FENG Jinghui, et al. Effects of Salvia miltiorrhiza Bunge extract and its single components on monosodium urate-induced pain in vivo and lipopolysaccharide-induced inflammation in vitro. J Tradit Chin Med. 2021. 41(2): 219-226.
