[CAS NO. 11103-72-3] Ruthenium red
PRODUCTS SPECIFICATIONS [11103-72-3]
DESCRIPTION [11103-72-3]
Overview
| MDL | MFCD00011479 |
|---|---|
| Molecular Weight | 786.35 |
| Molecular Formula | Cl6H42N14O2Ru3 |
| SMILES | [NH3][Ru](O[Ru]([NH3])([NH3])([NH3])([NH3])O[Ru]([NH3])([NH3])([NH3])([NH3])[NH3])([NH3])([NH3])([NH3])[NH3].[6+].[Cl-].[6] |
2 Publications Citing Use of MCE
Summary
Ruthenium red (Ammoniated ruthenium oxychloride) is a polycationic dye widely used for electron microscopy (EM) of cells, tissues and vegetative bacteria. Ruthenium red strongly reacts with phospholipids and fatty acids and binds to acidic mucopolysaccharides. Ruthenium red is a L-type calcium current ( I Ca ) blocker [1] [2] .
IC50 & Target
|
L-type calcium channel |
In Vitro
Ruthenium red staining of intracellular organelles and structures seemed to depend on the time of exposure to this agent, as well on the concentration used
[2]
.
Ruthenium red efficiently blocks the L-type calcium current in a dose-dependent manner in in guinea-pig isolated ventricular heart cells, with EC
50
of 0.8 μM
[2]
.
Ruthenium red (10 μM) blocks the sarcoplasmic Ca release channels or the mitochondrial Ca uptake, and blocks 26.7 % of the sodium current, and slows its inactivation time-course in guinea-pig isolated ventricular heart cells
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 10 mg/mL ( 12.72 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.2717 mL | 6.3585 mL | 12.7170 mL |
| 5 mM | 0.2543 mL | 1.2717 mL | 2.5434 mL |
| 10 mM | 0.1272 mL | 0.6358 mL | 1.2717 mL |
-
1.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: 1 mg/mL (1.27 mM); Clear solution; Need ultrasonic
References
- [1] Lashanda N Waller, et al. Ruthenium red staining for ultrastructural visualization of a glycoprotein layer surrounding the spore of Bacillus anthracis and Bacillus subtilis. J Microbiol Methods. 2004 Jul;58(1):23-30.
- [2] Claire O Malécot, et al. Ruthenium red as an effective blocker of calcium and sodium currents in guinea-pig isolated ventricular heart cells. Br J Pharmacol. 1998 Jun; 124(3): 465-472.
Synonyms
