[CAS NO. 117548-22-8] 5(6)-FAMSE
PRODUCTS SPECIFICATIONS [117548-22-8]
DESCRIPTION [117548-22-8]
Overview
| MDL | MFCD04119713 |
|---|---|
| Molecular Weight | 473.39 |
| Molecular Formula | C25H15NO9 |
| SMILES | OC1=CC=C2C(OC(C(C=C3)=C2C(C=CC(C(ON(C(CC4)=O)C4=O)=O)=C5)=C5C(O)=O)=CC3=O)=C1.OC6=CC=C7C(OC(C(C=C8)=C7C(C=C(C(ON(C(CC9)=O)C9=O)=O)C=C%10)=C%10C(O)=O)=CC8=O)=C6 |
Summary
5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 83.33 mg/mL ( 176.03 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.1124 mL | 10.5621 mL | 21.1242 mL |
| 5 mM | 0.4225 mL | 2.1124 mL | 4.2248 mL |
| 10 mM | 0.2112 mL | 1.0562 mL | 2.1124 mL |
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1.
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2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: 2.08 mg/mL (4.39 mM); Suspended solution; Need ultrasonic
References
- [1] Stafford JM, et al. Accessory factors facilitate the binding of glucocorticoid receptor to the phosphoenolpyruvate carboxykinase gene promoter. J Biol Chem. 2001 Oct 26;276(43):39885-91.
- [2] Powell RD, et al. Combined fluorescent and gold immunoprobes: reagents and methods for correlative light and electron microscopy. Microsc Res Tech. 1998 Jul 1;42(1):2-12.
