[CAS NO. 1239-45-8] Ethidium bromide

Name: Ethidium bromide
Brand: Arctom Scientific
SKU: AS658497

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PRODUCTS SPECIFICATIONS [1239-45-8]

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Catalog

AS658497

Brand

Arctom Scientific

CAS

1239-45-8

DESCRIPTION [1239-45-8]

Overview

MDL	MFCD00011724
Molecular Weight	394.31
Molecular Formula	C21H20N3.Br
SMILES	C(C)[N+]=1C(=C2C(=C3C1C=C(N)C=C3)C=CC(N)=C2)C4=CC=CC=C4.[Br-]

For research use only.

Storage

3 years,-20°C,powder
1 years,-80°C,in solvent

Shipping

Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)

Description

Ethidium Bromide (Homidium bromide, EtBr, EB) is an intercalating agent which resembles a DNA base pair and commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

In vitro

Ethidium Bromide (EtBr) is sometimes added to running buffer during the separation of DNA fragments by agarose gel electrophoresis. It is used because upon binding of the molecule to the DNA and illumination with a UV light source, the DNA banding pattern can be visualized. The mode of binding of EtBr is intercalation between the base pairs. This binding changes the charge, weight, conformation, and flexibility of the DNA molecule. The mobility of DNA was always less in the gels with EtBr.

References

[1] Sigmon J, et al. Electrophoresis. 1996, 17(10):1524-7.

Synonyms

Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1)

Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide

2,7-Diamino-9-phenyl-10-ethylphenanthridinium bromide

2,7-Diamino-9-phenylphenanthridine ethobromide

Dromilac

Ethidium bromide

Homidium bromide

2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide