[CAS NO. 1622006-09-0] Radioprotectin-1
PRODUCTS SPECIFICATIONS [1622006-09-0]
DESCRIPTION [1622006-09-0]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 486.92 |
| Molecular Formula | C23H19ClN2O6S |
| SMILES | O=C(O)C1=CC(Cl)=CC=C1S(=O)(NCCCCN(C(C2=CC=CC3=CC=CC4=C23)=O)C4=O)=O |
Summary
Radioprotectin-1 is a potent and specific nonlipid agonist of lysophosphatidic acid receptor 2 (LPA 2 ) , with an EC 50 value of 25 nM for murine LPA 2 subtype [1] .
IC50 & Target
EC50: 25 nM (murine LPA 2 subtype) [1]
In Vitro
Radioprotectin-1 is a potent agonist of LPA
2
with an EC
50
of 5 pM and functions as a full agonist at the human ortholog of LPA
2
[1]
.
Radioprotectin-1 (0-3 μM; 15 minutes) effectively reduces apoptosis induced by γ-irradiation and the radiomimetic drug Adriamycin in cells that expressed LPA
2
either endogenously or after transfection
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | MEF cells , IEC-6 cells |
| Concentration: | 0 μM, 0.1 μM, 0.3 μM, 1.0 μM,3 μM |
| Incubation Time: | 15 minutes |
| Result: | Protected cells from apoptosis by γ-irradiation- and the radiomimetic chemotherapeutic Adiamycin. |
In Vivo
Radioprotectin-1 is a high-potency specific agonist of the murine LPA
2
GPCR
[1]
.
Radioprotectin-1 (0.1 mg/kg, 0.3 mg/kg; s.c.; every 12 hours; for 3 days) decreases the mortality of C57BL/6 mice in models of the hematopoietic acute radiation syndromes (HE-ARS) and gastrointestinal acute radiation syndromes (GI-ARS)
[1]
.
Radioprotectin-1 exerts its radioprotective and radiomitigative action through specific activation of the upregulated LPA
2
GPCR in Lgr5
+
stem cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | 8-10 weeks old C57BL/6 female mice bearing total body irradiation (TBI) [1] |
| Dosage: | 0.1 mg/kg, 0.3 mg/kg |
| Administration: | Subcutaneous injection; every 12 hours; for 3 days |
| Result: | Decreased mortality of C57BL/6 mice in models of the HE-ARS and the GI-ARS. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 125 mg/mL ( 256.72 mM ; Need ultrasonic)
H 2 O : < 0.1 mg/mL (insoluble)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.0537 mL | 10.2686 mL | 20.5373 mL |
| 5 mM | 0.4107 mL | 2.0537 mL | 4.1075 mL |
| 10 mM | 0.2054 mL | 1.0269 mL | 2.0537 mL |
