[CAS NO. 1825355-56-3] Ribocil-C
PRODUCTS SPECIFICATIONS [1825355-56-3]
DESCRIPTION [1825355-56-3]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 419.50 |
| Molecular Formula | C21H21N7OS |
| SMILES | O=C1C=C(C2=CC=CS2)NC([C@H]3CCCN(CC4=CN(C5=NC=CC=N5)C=N4)C3)=N1 |
1 Publications Citing Use of MCE
Summary
Ribocil-C is a highly selective inhibitor of bacterial riboflavin riboswitches .
IC50 & Target
Bacterial riboflavin riboswitches [1]
In Vitro
Ribocil-C is a highly selective inhibitor of the flavin mononucleotide (FMN) riboswitch that controls expression of de novo riboflavin (RF, vitamin B2) biosynthesis in Escherichia coli . Ribocil-C specifically inhibits dual FMN riboswitches, separately controlling RF biosynthesis and uptake processes essential for Staphylococcus aureus growth and pathogenesis [1] . Ribocil-C is a small-molecule synthetic mimic of FMN that binds the FMN riboswitch of multiple GN bacteria, including Escherichia coli , Pseudomonas aeruginosa , and Acinetobacter baumannii , to inhibit ribB expression, RF synthesis, and consequently arrest bacterial growth [1] [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Higher dose Ribocil-C treatment groups (60 and 120 mg kg21 ribocil-C) demonstrate a dose-dependent reduction in bacterial burden of 1.87 and 3.29 log 10 [CFU per g spleen] reduction respectively versus shamtreated mice, without mortality or gross effects of toxicity observed [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 110 mg/mL ( 262.22 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.3838 mL | 11.9190 mL | 23.8379 mL |
| 5 mM | 0.4768 mL | 2.3838 mL | 4.7676 mL |
| 10 mM | 0.2384 mL | 1.1919 mL | 2.3838 mL |
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Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.75 mg/mL (6.56 mM); Clear solution
References
- [1] Wang H, et al. Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch DisruptRiboflavin Homeostasis in an Infectious Setting. Cell Chem Biol. 2017 May 18;24(5):576-588.
- [2] Howe JA, et al. Selective small-molecule inhibition of an RNA structural element. Nature. 2015 Oct 29;526(7575):672-7.
