[CAS NO. 1832686-44-8] SRI31215TFA
PRODUCTS SPECIFICATIONS [1832686-44-8]
DESCRIPTION [1832686-44-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 533.59 |
| Molecular Formula | C27H34F3N5O3 |
| SMILES | N=C(C1=CC=CC(N2CC(C)CN(CC3CCN(CC4=CC=CC=C4)CC3)C2=O)=C1)N.OC(C(F)(F)F)=O |
Summary
SRI 31215 (TFA) is a Matriptase/Hepsin/hepatocyte growth factor activator (HGFA) triplex inhibitor and mimics the activity of HAI-1/2 (endogenous inhibitors of HGF activation). SRI 31215 has potent inhibitory activity against matriptase, hepsin and HGFA with IC 50 values of 0.69 μM, 0.65 μM and 0.30 μM, respectively. SRI 31215 can be used for the research of cancer [1] .
IC50 & Target
IC50: 0.69 μM (matriptase); 0.65 μM (hepsin); 0.30 μM (HGFA) [1]
In Vitro
SRI 31215 (TFA) has potent activity against matriptase, hepsin and HGFA with
IC
50
values of 0.69 μM, 0.65 μM and 0.30 μM, respectively
[1]
.
SRI 31215 (10 μM) inhibits the proteolytic activation of pro-HGF
[1]
.
SRI 31215 (10 μM, 30 min) inhibits fibroblast-induced HGF/MET signaling in tumor cells
[1]
.
SRI 31215 (10 μM, 24 h) inhibits fibroblast-induced epithelial mesenchymal transition (EMT) and migration in tumor cells
[1]
.
SRI 31215 (10 μM) overcomes the resistance to EGFR inhibitors mediated by autocrine HGF/MET signaling in colon cancer cells
[1]
.
SRI31215 (10 μM, 72 h) averts fibroblast-mediated resistance to EGFRi-induced apoptosis
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | DU145 cells |
| Concentration: | 10 μM |
| Incubation Time: | 30 min |
| Result: | Prevented fibroblast-induced MET activation and signaling in tumor cells, but did not prevent MET activation induced by active HGF. |
Cell Migration Assay [1]
| Cell Line: | DU145 cells |
| Concentration: | 10 μM |
| Incubation Time: | 24 h |
| Result: | Did not interfere with HGF-induced migration, but inhibited fibroblast-induced migration of DU145 cells. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 125 mg/mL ( 234.26 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.8741 mL | 9.3705 mL | 18.7410 mL |
| 5 mM | 0.3748 mL | 1.8741 mL | 3.7482 mL |
| 10 mM | 0.1874 mL | 0.9370 mL | 1.8741 mL |
