[CAS NO. 2064292-12-0] A1874
PRODUCTS SPECIFICATIONS [2064292-12-0]
DESCRIPTION [2064292-12-0]
Overview
| MDL | MFCD31813733 |
|---|---|
| Molecular Weight | 1173.59 |
| Molecular Formula | C58H62Cl3F2N9O7S |
| SMILES | ClC(C=C1)=CC=C1C2=N[C@H](C3=NN=C(C)N3C4=C2C(C)=C(C)S4)CC(NCCOCCOCCOCCNC(C5=CC(OC)=C(NC([C@H]6[C@H](C7=C(F)C(Cl)=CC=C7)[C@](C8=CC=C(Cl)C=C8F)(C#N)[C@H](CC(C)(C)C)N6)=O)C=C5)=O)=O |
Summary
IC50 & Target
|
BRD4 |
In Vitro
Treatment of HCT116 cells of A1874 (0-10 μM, 24 hours) induces a dose-dependent knockdown of BRD4 levels, with near-maximum knockdown by 100 nmol/L and a maximum degradation (Dmax) of BRD4 of 98% of the levels in control (0.1% DMSO-treated) cells
[1]
.
Treatment of HCT116 cells of A1874 (0-10 μM, 24 hours) increases p53 levels in the HCT116 cells and showed dose-dependent p53 stabilization
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | HCT116 cells. |
| Concentration: | 0-10 μM. |
| Incubation Time: | 24 hours. |
| Result: |
Induced a dosedependent knockdown of BRD4 levels, with near-maximum knockdown by 100 nM.
Increased p53 levels and showed dose-dependent p53 stabilization. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 150 mg/mL ( 127.81 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 0.8521 mL | 4.2604 mL | 8.5209 mL |
| 5 mM | 0.1704 mL | 0.8521 mL | 1.7042 mL |
| 10 mM | 0.0852 mL | 0.4260 mL | 0.8521 mL |
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1.
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2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (2.13 mM); Clear solution
