[CAS NO. 2407957-87-1] EGFR-IN-8
PRODUCTS SPECIFICATIONS [2407957-87-1]
DESCRIPTION [2407957-87-1]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 662.02 |
| Molecular Formula | C32H23ClF3N7O4 |
| SMILES | O=C(NC1=CC(C(F)(F)F)=C(NC(C2CC2)=O)C=C1)NC3=CC=CC(C4=NC(C5=CC=C(NC(C6=CC=CN=C6)=O)C=C5Cl)=NO4)=C3 |
Summary
IC50 & Target
EGFR; c-Met [1]
In Vitro
EGFR-IN-8 (0-20 μM; 24-72 hours) exhibits a time- and dose-dependent inhibitory effect on the viability of A549, PC9, H1975, CL68, and CL97 cells at different time intervals, with IC
50
values ranging from 0.3 to 0.6 μM and 0.2-0.5 μM after 48 and 72 h of treatment respectively
[1]
.
EGFR-IN-8 (0-0.6 μM; 48 hours) suppresses the expression of EGFR and c-Met in these five cell lines irrespective of their mutational status
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | A549, PC9, H1975, CL68, and CL97 cells |
| Concentration: | 0-20 μM |
| Incubation Time: | 24 hours, 48 hours, 72 hours |
| Result: | Showed inhibitory effects on different cells. |
Western Blot Analysis [1]
| Cell Line: | A549, PC9, H1975, CL68, and CL97 cells |
| Concentration: | 0-0.6 μM |
| Incubation Time: | 48 hours |
| Result: | Decreased EGFR and c-Met expression. |
In Vivo
EGFR-IN-8 (oral gavage; 50 and 150 mg/kg; once daily; 20 days) exhibits a dose-dependent suppression (29% and 60%, respectively) of H1975 xenograft tumor growth at 50 and 150 mg/kg
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Nude mice H1975 xenograft tumor model [1] |
| Dosage: | 50 and 150 mg/kg |
| Administration: | Oral gavage; 50 and 150 mg/kg; once daily; 20 days |
| Result: | Inhibited H1975 xenograft tumor growth. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 8.33 mg/mL ( 12.58 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.5105 mL | 7.5526 mL | 15.1053 mL |
| 5 mM | 0.3021 mL | 1.5105 mL | 3.0211 mL |
| 10 mM | 0.1511 mL | 0.7553 mL | 1.5105 mL |
