[CAS NO. 281224-40-6] M-31850
PRODUCTS SPECIFICATIONS [281224-40-6]
DESCRIPTION [281224-40-6]
Overview
| MDL | MFCD00238908 |
|---|---|
| Molecular Weight | 463.48 |
| Molecular Formula | C28H21N3O4 |
| SMILES | O=C(C1=CC=CC2=C1C3=CC=C2)N(CCNCCN4C(C5=CC=CC6=C5C(C4=O)=CC=C6)=O)C3=O |
Summary
In Vitro
M-31850 shows some activity towards Jack Bean Hex (JBHex) and bacterial Hex from
Streptomyces plicatus
(SpHex) (IC
50
of 280 µM and >500 µM for JBHex and SpHex, respectively)
[1]
.
M-31850 produces a dose dependent increase in MUG hydrolysis (Hex S levels) in lysates from treated infantile Sandhoff disease (ISD) cells
[1]
.
M-31850 increases the half-life of the mutant Hex A from Adult forms of Tay-Sachs (ATSD) cells more than two-fold at 44° C, relative to the enzyme heated in the presence of DMSO. M-31850 acts as a classic competitive inhibitor of Hex (K
m
increases and V
max
is unaffected by increasing amounts of M-31850), with a K
i
of 0.8 μM
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 5 mg/mL ( 10.79 mM ; ultrasonic and adjust pH to 6 with HCl)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.1576 mL | 10.7880 mL | 21.5759 mL |
| 5 mM | 0.4315 mL | 2.1576 mL | 4.3152 mL |
| 10 mM | 0.2158 mL | 1.0788 mL | 2.1576 mL |
References
- [1] Michael B Tropak, et al. High-throughput screening for human lysosomal beta-N-Acetyl hexosaminidase inhibitors acting as pharmacological chaperones. Chem Biol. 2007 Feb;14(2):153-64.
- [2] Qi Chen, et al. Exploring unsymmetrical dyads as efficient inhibitors against the insect β-N-acetyl-D-hexosaminidase OfHex2. Biochimie. 2014 Feb;97:152-62.
