[CAS NO. 3031-95-6] SAICAR

Name: SAICAR
Brand: Arctom Scientific
SKU: AS021398

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PRODUCTS SPECIFICATIONS [3031-95-6]

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Catalog

AS021398

Brand

Arctom Scientific

CAS

3031-95-6

DESCRIPTION [3031-95-6]

Overview

MDL	-
Molecular Weight	454.28
Molecular Formula	C13H19N4O12P
SMILES	O=C(O)C[C@@H](C(O)=O)NC(C1=C(N)N([C@H]2[C@@H]([C@@H]([C@@H](COP(O)(O)=O)O2)O)O)C=N1)=O

For research use only. We do not sell to patients.

1 Publications Citing Use of MCE

[1]Drug Test Anal. 2022 Nov 7.

Summary

SAICAR is an intermediate of de novo purine nucleotide biosynthesis, activates pyruvate kinase isoform M2 (PKM2) in an isozyme-selective manner, with an EC ₅₀ of 0.3 mM. SAICAR stimulates PKM2 and promotes cancer cell survival in glucose-limited conditions ^[1] ^[2] .

IC50 & Target

Human Endogenous Metabolite

In Vitro

SAICAR accumulation induces nuclear localization of PKM2. PKM2-SAICAR phosphorylates and activates Erk1/2, which in turn sensitizes PKM2 for SAICAR binding through phosphorylation. Additionally, PKM2-SAICAR was necessary to induce sustained Erk1/2 activation and mitogen-induced cell proliferation. SAICAR-PKM2 interaction is necessary and sufficient to induce H3 T11 and Erk1/2 phosphorylation ^[2] .
Upon glucose starvation, cellular SAICAR concentration increases in an oscillatory manner and stimulates PKM2 activity in cancer cells. The SAICAR-PKM2 interaction also promotes cancer cell survival in glucose-limited conditions. In glucose-limited conditions, cells with higher SAICAR concentrations ( adsl-kd cells or cells overexpressing PAICS) survive better while paics-kd cells died earlier than control-kd cells. SAICAR promotes cancer cell survival in glucose-limited conditions ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 220 mg/mL ( 484.28 mM ; Need ultrasonic)

H ₂ O : 100 mg/mL ( 220.13 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2013 mL	11.0064 mL	22.0129 mL
5 mM	0.4403 mL	2.2013 mL	4.4026 mL
10 mM	0.2201 mL	1.1006 mL	2.2013 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 5.5 mg/mL (12.11 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 5.5 mg/mL (12.11 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 5.5 mg/mL (12.11 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

L-Aspartic acid, N-[[5-amino-1-(5-O-phosphono-β-D-ribofuranosyl)-1H-imidazol-4-yl]carbonyl]-

Aspartic acid, N-[(5-amino-1-β-D-ribofuranosylimidazol-4-yl)carbonyl]-, 5′-(dihydrogen phosphate), L-

N-[[5-Amino-1-(5-O-phosphono-β-D-ribofuranosyl)-1H-imidazol-4-yl]carbonyl]-L-aspartic acid

N-[5-Amino-1-(5′-phosphoribofuranosyl)-4-imidazolecarbonyl]aspartic acid

N-(5-Amino-1-ribofuranosylimidazol-4-ylcarbonyl)aspartic acid 5′-phosphate

SAICAR

Succino-AICAR

5-Amino-4-imidazole-N-succinocarboxamide ribonucleotide