[CAS NO. 351986-85-1] Vacuolin-1

Name: Vacuolin-1
Brand: Arctom Scientific
SKU: AS079183

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PRODUCTS SPECIFICATIONS [351986-85-1]

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Catalog

AS079183

Brand

Arctom Scientific

CAS

351986-85-1

DESCRIPTION [351986-85-1]

Overview

MDL	MFCD00321410
Molecular Weight	577.42
Molecular Formula	C26H24IN7O
SMILES	IC1=CC(/C=N/NC2=NC(N(C3=CC=CC=C3)C4=CC=CC=C4)=NC(N5CCOCC5)=N2)=CC=C1

For research use only. We do not sell to patients.

1 Publications Citing Use of MCE

[1]Basic Res Cardiol. 2022 Apr 7;117(1):20.

Summary

Vacuolin-1 is a potent and cell-permeable lysosomal exocytosis inhibitor. Vacuolin-1 blocks the Ca ²⁺ -dependent exocytosis of lysosomes and prevents the release of lysosomal content without affecting the process of resealing. vacuolin‐1 is a potent and selective PIKfyve inhibitor and inhibits late‐stage autophagy by impairing lysosomal maturation ^[1] ^[2] .

In Vitro

Vacuolin-1 (1 μM; Pretreatment 1 hour) blocks ionomycin-induced exocytosis of lysosomes but not of enlargeosomes. It results in vacuolation of lysosomes (A) and complete inhibition of the surface expression of Lamp-1 ^[1] .
Vacuolin-1 (1 μM; 20-180 min) disrupts the segregation of inner and limiting membranes characteristic of endosomes and lysosomes. Ultrastructural analysis also shows that vacuolin-1 favours fusion of inner and limiting membranes ^[1] .
Vacuolin-1 (5 or 10 μM; 2 hours) blocks the Ca ²⁺ -dependent release of β-hexosaminidase from lysosomes. In HeLa cells, ionomycin results in the expected release of 18-20% of lysosomal β-hexosaminidase. But when cells pretreated with vacuolin-1, releases no more β-hexosaminidase compared with cells that are not exposed to ionomycin (4%) ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL ( 17.32 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7318 mL	8.6592 mL	17.3184 mL
5 mM	0.3464 mL	1.7318 mL	3.4637 mL
10 mM	0.1732 mL	0.8659 mL	1.7318 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: 1 mg/mL (1.73 mM); Suspended solution; Need ultrasonic
2.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 1 mg/mL (1.73 mM); Clear solution

* All of the co-solvents are available by MCE.