[CAS NO. 4272-74-6] N-alpha-Tosyl-L-lysinechloromethylketonehydrochloride

Name: N-alpha-Tosyl-L-lysinechloromethylketonehydrochloride
Brand: Arctom Scientific
SKU: AS169385

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PRODUCTS SPECIFICATIONS [4272-74-6]

Distributor

Catalog

AS169385

Brand

Arctom Scientific

CAS

4272-74-6

DESCRIPTION [4272-74-6]

Overview

MDL	MFCD00065395
Molecular Weight	369.31
Molecular Formula	C14H22Cl2N2O3S
SMILES	O=S(C1=CC=C(C)C=C1)(N[C@H](C(CCl)=O)CCCCN)=O.Cl

For research use only. We do not sell to patients.

1 Publications Citing Use of MCE

[1]Cell Death Dis. 2021 Jan 7;12(1):42.

Summary

N-alpha-Tosyl-L-lysine chloromethyl ketone (TLCK), a trypsin like protease inhibitor, sensitizes HeLa cells to Fas-mediated cell death.

In Vitro

N-alpha-Tosyl-L-lysine chloromethyl ketone exhibits an inhibitory effect on IFN-γ activities. The effect of TLCK is studied on the IFN-γ sensitization of HeLa cells towards cell death mediated by anti-Fas. Lower concentration of anti-Fas (10 ng/mL) are used to examine the interaction among the three effectors simultaneously, that is, anti-Fas, TLCK and IFN-γ. TLCK by itself up to 50 μM concentration exhibits a small decrease in cell viability. Beyond 50 μM, a dose dependent decrease in cell viability is observed. IFN-γ slightly reduces cell viability on its own. Addition of anti-Fas (10 ng/mL) results in a slight decrease in cell survival, which is enhanced more than additively in the presence of TLCK, most prominently between 50 and 100 μM. Upon addition of both anti-Fas and IFN-γ, a decrease (≈46%) in cell viability is observed. Moreover, the decrease in cell survival is further enhanced upon addition of higher concentrations of TLCK, 25 μM and more ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL ( 676.94 mM ; Need ultrasonic)

H ₂ O : 100 mg/mL ( 270.78 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7078 mL	13.5388 mL	27.0775 mL
5 mM	0.5416 mL	2.7078 mL	5.4155 mL
10 mM	0.2708 mL	1.3539 mL	2.7078 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (5.63 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (5.63 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.08 mg/mL (5.63 mM); Clear solution

* All of the co-solvents are available by MCE.

References

[1] Shadrin N, et al. Serine protease inhibitors interact with IFN-γ through up-regulation of FasR; a novel therapeutic strategy against cancer. Exp Cell Res. 2015 Jan 15;330(2):233-9.

Synonyms

Benzenesulfonamide, N-[(1S)-5-amino-1-(2-chloroacetyl)pentyl]-4-methyl-, hydrochloride (1:1)

p-Toluenesulfonamide, N-[5-amino-1-(chloroacetyl)pentyl]-, monohydrochloride, L-

Benzenesulfonamide, N-[5-amino-1-(chloroacetyl)pentyl]-4-methyl-, monohydrochloride, (S)-

Benzenesulfonamide, N-[(1S)-5-amino-1-(chloroacetyl)pentyl]-4-methyl-, monohydrochloride

N^α-p-Tosyl-L-lysine chloromethyl ketone hydrochloride

N-α-Tosyl-L-lysylchloromethane hydrochloride

(S)-N-(7-Amino-1-chloro-2-oxoheptan-3-yl)-4-methylbenzenesulfonamide hydrochloride