[CAS NO. 4386-25-8] Lumogallion
PRODUCTS SPECIFICATIONS [4386-25-8]
DESCRIPTION [4386-25-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 344.73 |
| Molecular Formula | C12H9ClN2O6S |
| SMILES | O=S(C1=CC(Cl)=CC(/N=N/C2=CC=C(O)C=C2O)=C1O)(O)=O |
Summary
In Vitro
Lumogallion (1 mM, 45 min) can be used to detect aluminum levels in brain tissue of Alzheimer's patients
[1]
.
Lumogallion can be used as a probe to stain live cells with intracellular aluminum adjuvant
[2]
.
Lumogallion (10 μM, 60 min) can be used to detect aluminum content in root tip cell tissue of plants
[3]
.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolved lumogallion in 0.1 M acetate buffer (pH 5.2).
2. Sample and 10 μM lumogallion were co-incubated for 60 min in the dark.
3. Washed the mixed sample well with acetate buffer solution twice, 15 minutes each, and observed under a confocal laser microscope.
4. Excitation wavelength is 488 nm , emission wavelength is 520 nm.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL ( 290.08 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.9008 mL | 14.5041 mL | 29.0082 mL |
| 5 mM | 0.5802 mL | 2.9008 mL | 5.8016 mL |
| 10 mM | 0.2901 mL | 1.4504 mL | 2.9008 mL |
References
- [1] Ambreen Mirza, et al. The Identification of Aluminum in Human Brain Tissue Using Lumogallion and Fluorescence Microscopy. J Alzheimers Dis. 2016 Oct 18;54(4):1333-1338.
- [2] Irene Mile, et al. Al adjuvants can be tracked in viable cells by lumogallion staining. J Immunol Methods. 2015 Jul;422:87-94.
- [3] Tatsuhiko Kataoka, et al. Highly sensitive analytical method for aluminum movement in soybean root through lumogallion staining. J. Plant Res. 110, 305–309 (1997).
Synonyms
