[CAS NO. 484-78-6] 3-Hydroxykynurenine
PRODUCTS SPECIFICATIONS [484-78-6]
DESCRIPTION [484-78-6]
Overview
| MDL | MFCD00047832 |
|---|---|
| Molecular Weight | 224.21 |
| Molecular Formula | C10H12N2O4 |
| SMILES | O=C(O)C(N)CC(C1=CC=CC(O)=C1N)=O |
Summary
IC50 & Target
|
Human Endogenous Metabolite |
In Vitro
3-Hydroxykynurenine-induced neuronal cell death shows several features of apoptosis
[1]
.
3-Hydroxykynurenine is a metabolic intermediate of the kynurenine pathway, the major metabolic pathway of tryptophan (Trp)
[1]
.
3-Hydroxykynurenine acts as an endogenous neurotoxin in the brain under pathological conditions
[1]
.
3-Hydroxykynurenine (20-100 μM) significantly inhibits CD4
+
T-cell proliferation in a dose-dependent manner with IC
50
of approximately 70 μM
[2]
.
3-Hydroxykynurenine (20-100 μM) induces significant CD4
+
T-cell-mediated cell death in a dose-dependent manner
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [2]
| Cell Line: | CD3/CD28 bead-stimulated CD4 + T cells |
| Concentration: | 0, 20, 40, 60, 80, and 100 μM |
| Incubation Time: | 72 hours |
| Result: | Significantly inhibited CD4 + T-cell proliferation in a dose-dependent manner with an IC 50 of approximately 70 μM. |
In Vivo
3-Hydroxykynurenine administration (560 mg/kg IP on a daily basis on days 1 to 7, days 7 to 14, or days 1 to 14) results in significant prolongation of graft survival whether administered between days 1 to 7, days 7 to 14, or days 1 to 14 [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | BALB/c (H2d) mice [2] |
| Dosage: | 560 mg/kg |
| Administration: | IP injection |
| Result: | There was significant prolongation of graft survival in all treatment groups in comparison to the controls. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
1M HCl : 33.33 mg/mL ( 148.66 mM ; ultrasonic and adjust pH to 1 with HCl)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.4601 mL | 22.3005 mL | 44.6010 mL |
| 5 mM | 0.8920 mL | 4.4601 mL | 8.9202 mL |
| 10 mM | 0.4460 mL | 2.2301 mL | 4.4601 mL |
References
- [1] S Okuda, et al. 3-Hydroxykynurenine, an endogenous oxidative stress generator, causes neuronal cell death with apoptotic features and region selectivity. J Neurochem. 1998 Jan;70(1):299-307.
- [2] Sarah S Zaher,et al. 3-hydroxykynurenine suppresses CD4+ T-cell proliferation, induces T-regulatory-cell development, and prolongs corneal allograft survival. Invest Ophthalmol Vis Sci. 2011 Apr 22;52(5):2640-8.
Synonyms
