[CAS NO. 5142-23-4] 3-Methyladenine

Name: 3-Methyladenine
Brand: Arctom Scientific
SKU: AS016753

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PRODUCTS SPECIFICATIONS [5142-23-4]

Store

Catalog

AS016753

Brand

Arctom Scientific

CAS

5142-23-4

DESCRIPTION [5142-23-4]

Overview

MDL	MFCD00010531
Molecular Weight	149.15
Molecular Formula	C6H7N5
SMILES	NC1=C2N=CN=C2N(C)C=N1

For research use only. We do not sell to patients.

458 Publications Citing Use of MCE

Summary

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K ^[1] .

IC50 & Target

PtdIns3Kγ

60 μM (IC ₅₀ , Cell Assay)

Vps34

25 μM (IC ₅₀ , Cell Assay)

Autophagy

Mitophagy

Human Endogenous Metabolite

In Vitro

3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner ^[2] .
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions ^[2] .
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy ^[2] .
3-Methyladenine induces cell death is independent of autophagy inhibition ^[2] .
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest ^[2] .
Note:
The recommended concentration of 3-MA is approximately 0.5-10 mM in cell culture. DMSO stock solution is not recommended. We suggest that you can weigh out the amount of 3-MA you required before your experiment. Then please dissolve it in medium and sterilize with a 0.22 μm filter.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay ^[2]

Cell Line:	HeLa cells
Concentration:	0 mM, 2.5 mM or 5 mM, 10 mM
Incubation Time:	0 hour, 24 hours and 48 hours
Result:	Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation.

Cell Autophagy Assay ^[2]

Cell Line:	HeLa cells
Concentration:	5 mM
Incubation Time:	24 hours
Result:	Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions.

Western Blot Analysis ^[2]

Cell Line:	HeLa cells
Concentration:	5 mM
Incubation Time:	0 hour, 12 hours, 24 hours and 48 hours
Result:	Suppressed conversion of LC3-I to LC3-II between 12 hours and 48 hours.

In Vivo

3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours ^[3] .
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP ^[3] .
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290 g) ^[3]
Dosage:	1.5 mg/100 g (1000 μM)
Administration:	Intraperitoneal injection
Result:	Alleviated Sodium Taurocholate-Induced SAP.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H ₂ O : 5 mg/mL ( 33.52 mM ; Need ultrasonic)

DMSO : 8.33 mg/mL ( 55.85 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.7047 mL	33.5233 mL	67.0466 mL
5 mM	1.3409 mL	6.7047 mL	13.4093 mL
10 mM	0.6705 mL	3.3523 mL	6.7047 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: PBS

Solubility: 4 mg/mL (26.82 mM); Clear solution; Need ultrasonic

* All of the co-solvents are available by MCE.

References

Synonyms

3H-Purin-6-amine, 3-methyl-

Adenine, 3-methyl-

3-Methyl-3H-purin-6-amine

3-Methyladenine

N³-Methyladenine

6-Amino-3-methylpurine

3-Methyl-6-aminopurine

NSC 66389

3-Methyl-3H-purin-6-ylamine

3-Methylpurin-6-amine