[CAS NO. 5168-36-5] 5-Hydroxy-2'-deoxyuridine
PRODUCTS SPECIFICATIONS [5168-36-5]
DESCRIPTION [5168-36-5]
Overview
| MDL | MFCD00057414 |
|---|---|
| Molecular Weight | 244.20 |
| Molecular Formula | C9H12N2O6 |
| SMILES | OC[C@@H]1[C@H](C[C@H](N2C(NC(C(O)=C2)=O)=O)O1)O |
Summary
5-Hydroxy-2'-deoxyuridine (5-OHdU) is a major stable oxidation product of 2'-Deoxycytidine. 5-Hydroxy-2'-deoxyuridine can be incorporated into DNA in vitro by DNA polymerase [1] .
IC50 & Target
|
Human Endogenous Metabolite |
In Vitro
To study the specificity of nucleotide incorporation opposite 5-hydroxypyrimidines in template DNA, 18- and 45-member oligodeoxyribonucleotides, containing an internal 2'-deoxy-5-hydroxyuridine (5-OHdU) in two different sequence contexts, were used. Translesion synthesis past 2'-deoxy-5-hydroxyuridine (5-OHdU) in both oligonucleotides occurred, but pauses both opposite, and one nucleotide prior to, the modified base in the template is observed. The specificity of nucleotide incorporation opposite 2'-deoxy-5-hydroxyuridine (5-OHdU) in the template is sequence context dependent. In one sequence context, dA is the principal nucleotide incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU). However in a second sequence context, dC is the predominant base incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU). In that same sequence context, dC is also the predominant nucleotide incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU) [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 33.33 mg/mL ( 136.49 mM ; Need ultrasonic)
H 2 O : 10 mg/mL ( 40.95 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.0950 mL | 20.4750 mL | 40.9500 mL |
| 5 mM | 0.8190 mL | 4.0950 mL | 8.1900 mL |
| 10 mM | 0.4095 mL | 2.0475 mL | 4.0950 mL |
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1.
Add each solvent one by one: PBS
Solubility: 10 mg/mL (40.95 mM); Clear solution; Need ultrasonic
-
2.
-
3.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: 2 mg/mL (8.19 mM); Clear solution; Need ultrasonic
-
4.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: 2 mg/mL (8.19 mM); Clear solution; Need ultrasonic
