[CAS NO. 52811-37-7] Cearoin
PRODUCTS SPECIFICATIONS [52811-37-7]
DESCRIPTION [52811-37-7]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 244.24 |
| Molecular Formula | C14H12O4 |
| SMILES | O=C(C1=CC(O)=C(OC)C=C1O)C2=CC=CC=C2 |
Summary
IC50 & Target
|
ERK |
ROS |
Autophagy |
Apoptosis |
In Vitro
Cearoin (10-80 μM) induces cell death in a dose-dependent manner
[1]
.
Cearoin (10-80 μM) increases the phosporylation of ERK in SH-SY5Y cells
[1]
.
Cearoin (5-80 μM) increases the conversion of LC3B-I to LC3B-II in SH-SY5Y cells. The expression of LC3B-II is a good marker for autophagosome formation in the autophagy process
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | Human neuroblastoma SH-SY5Y cells |
| Concentration: | 0, 1, 5, 10, 20, 40, or 80 μM |
| Incubation Time: | 6 or 12 hours |
| Result: | Significantly decreased cell viability from 10 μM in a dose-dependent manner. Treatment with 40 μM for 12 h induced about 50% loss in cell viability in SH-SY5Y cells. |
Western Blot Analysis [1]
| Cell Line: | Human neuroblastoma SH-SY5Y cells |
| Concentration: | 0, 5, 10, 20, 40, or 80 μM |
| Incubation Time: | 12 hours |
| Result: |
Increased ERK phosphorylation in a dose-dependent manner, whereas it did not alter JNK phosphorylation.
Induced the formation of LC3B-II in a dose dependent manner. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL ( 204.72 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.0943 mL | 20.4717 mL | 40.9433 mL |
| 5 mM | 0.8189 mL | 4.0943 mL | 8.1887 mL |
| 10 mM | 0.4094 mL | 2.0472 mL | 4.0943 mL |
