[CAS NO. 551-11-1] Dinoprost
PRODUCTS SPECIFICATIONS [551-11-1]
DESCRIPTION [551-11-1]
Overview
| MDL | MFCD00135231 |
|---|---|
| Molecular Weight | 354.48 |
| Molecular Formula | C20H34O5 |
| SMILES | CCCCC[C@H](O)/C=C/[C@H]1[C@H](O)C[C@H](O)[C@@H]1C/C=C\CCCC(O)=O |
Summary
Dinoprost (Prostaglandin F2α) is an orally active, potent prostaglandin F (PGF) receptor (FP receptor) agonist. Dinoprost is a luteolytic hormone produced locally in the endometrial luminal epithelium and corpus luteum (CL). Dinoprost plays a key role in the onset and progression of labour [1] [2] .
IC50 & Target
|
FP Receptor |
Human Endogenous Metabolite |
In Vitro
Dinoprost (Prostaglandin F2α; 1 μM; for 24 hours) induces ER stress, autophagy, and apoptosis in goat luteal cells
[1]
.
Dinoprost (1 μM; for 24 hours) significantly increases the expression of GRP78 and UPR sensors
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Apoptosis Analysis [1]
| Cell Line: | Goat luteal cells |
| Concentration: | 1 μM |
| Incubation Time: | For 24 hours |
| Result: | Significantly increased the apoptotic rate (15.62±3.12%). |
Cell Autophagy Assay [1]
| Cell Line: | Goat luteal cells |
| Concentration: | 1 μM |
| Incubation Time: | For 24 hours |
| Result: | There was extensive overlap between LC3 and LAMP1 in luteal cells and autophagolysosomes were formed in goat luteal cells. |
Western Blot Analysis [1]
| Cell Line: | Goat luteal cells |
| Concentration: | 1 μM |
| Incubation Time: | For 24 hours |
| Result: | The expression of GRP78 and UPR sensors including cleaved ATF6, phosphorylated-EIF2S1, EIF2S1, ATF4, phosphorylated-IRE1, autophagy-related protein LC3-II, and pro-apoptosis factor cleaved Caspase3 increased significantly in the cells. |
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date | Phase |
|---|---|---|---|---|
| NCT03584854 | Brigham and Women´s Hospital |
Postpartum Hemorrhage|Uterine Atony
|
March 1, 2019 | Phase 4 |
Appearance
<25°C Solid,>35°C Liquid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Pure form | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 282.10 mM ; Need ultrasonic)
H 2 O : 100 mg/mL ( 282.10 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.8210 mL | 14.1052 mL | 28.2103 mL |
| 5 mM | 0.5642 mL | 2.8210 mL | 5.6421 mL |
| 10 mM | 0.2821 mL | 1.4105 mL | 2.8210 mL |
-
1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (7.05 mM); Clear solution
References
- [1] Hagen Thieme, et al. Endothelin antagonism: effects of FP receptor agonists prostaglandin F2alpha and fluprostenol on trabecular meshwork contractility. Invest Ophthalmol Vis Sci. 2006 Mar;47(3):938-45.
- [2] Xin Wen, et al. Prostaglandin F2α Induces Goat Corpus Luteum Regression via Endoplasmic Reticulum Stress and Autophagy. Front Physiol. 2020 Sep 11;11:868.
Synonyms
