[CAS NO. 607737-87-1] A77-01
PRODUCTS SPECIFICATIONS [607737-87-1]
DESCRIPTION [607737-87-1]
Overview
| MDL | MFCD07367791 |
|---|---|
| Molecular Weight | 286.33 |
| Molecular Formula | C18H14N4 |
| SMILES | CC1=CC=CC(C2=NNC=C2C3=CC=NC4=CC=CC=C34)=N1 |
1 Publications Citing Use of MCE
Summary
IC50 & Target
IC50: 25 nM (ALK5) [1]
In Vitro
A-77-01 (0.01-10 µM; 25 hours; Mv1Lu cells) treatment effectively inhibits TGF-β-induced transcriptional activation in concentration-dependent fashion
[1]
.
A-77-01 (1 µM; 24-72 hours; Mv1Lu cells) treatment efficiently prevents the growth-inhibitory effects of TGF-β
[1]
.
A-77-01 (1 µM; 30 minutes; HaCaT cells) treatment inhibits TGF-β-induced phosphorylation of Smad2
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
RT-PCR [1]
| Cell Line: | Mv1Lu cells |
| Concentration: | 0.01 µM, 0.03 µM, 0.1 µM, 0.3 µM, 1 µM, 3 µM, 10 µM |
| Incubation Time: | 25 hours |
| Result: | Inhibited the luciferase activity induced by TGF-β in concentration-dependent fashion. |
Cell Viability Assay [1]
| Cell Line: | Mv1Lu cells |
| Concentration: | 1 µM |
| Incubation Time: | 24 hours, 48 hours, 72 hours |
| Result: | Efficiently prevented the growth-inhibitory effects of TGF-β. |
Western Blot Analysis [1]
| Cell Line: | HaCaT cells |
| Concentration: | 1 µM |
| Incubation Time: | 30 minutes |
| Result: | Inhibited TGF-β-induced phosphorylation of Smad2. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 25 mg/mL ( 87.31 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.4925 mL | 17.4624 mL | 34.9247 mL |
| 5 mM | 0.6985 mL | 3.4925 mL | 6.9849 mL |
| 10 mM | 0.3492 mL | 1.7462 mL | 3.4925 mL |
