[CAS NO. 614-05-1] Oxythiamine chloride hydrochloride
PRODUCTS SPECIFICATIONS [614-05-1]
DESCRIPTION [614-05-1]
Overview
| MDL | MFCD00050656 |
|---|---|
| Molecular Weight | 338.25 |
| Molecular Formula | C12H17Cl2N3O2S |
| SMILES | CC1=C(CCO)SC=[N+]1CC2=CN=C(C)NC2=O.Cl.[Cl-] |
1 Publications Citing Use of MCE
Summary
Oxythiamine (Hydroxythiamine) chloride hydrochloride, an analogue of anti-metabolite, can suppress the non-oxidative synthesis of ribose and induce cell apoptosis . Oxythiamine chloride hydrochloride is a thiamine antagonist and inhibits transketolase (TK). Oxythiamine chloride hydrochloride inhibits cancer cell apoptosis and inhibits cell proliferation [1] [2] [3] .
IC50 & Target
Vitamin B1, Thiamine, Transketolase [1] .
In Vitro
Oxythiamine chloride hydrochloride (0-40 μM, 2 days) inhibits cell viability of MIA PaCa-2 cells (IC
50
: 14.95 μM)
[1]
.
Oxythiamine chloride hydrochloride (0-500 μM, 48 h) suppresses expression of 14-3-3 protein beta/alpha in MIA PaCa-2 cells
[1]
.
Oxythiamine chloride hydrochloride (0.1-100 μM, 6-48 h) inhibits A549 cell proliferation
[3]
.
Oxythiamine chloride hydrochloride (0.1-100 μM, 24 h) induces A549 cell apoptosis
[3]
.
Oxythiamine chloride hydrochloride (0–20 μM) inhibits the invasion and migration (IC
50
: 8.75 μM) of Lewis lung carcinoma (LLC) cells
[4]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | MIA PaCa-2 cells |
| Concentration: | 0-40 μM |
| Incubation Time: | 2 days |
| Result: | Inhibited cell viability with an IC 50 of 14.95 μM. |
Western Blot Analysis [1]
| Cell Line: | MIA PaCa-2 cells |
| Concentration: | 0, 5, 50, 500 μM |
| Incubation Time: | 48 h |
| Result: | Inhibited 14-3-3 protein beta/alpha expression, and increased alpha-enolase. |
In Vivo
Oxythiamine chloride hydrochloride (100-500 mg/kg, i.p. 4 days) inhibits tumor growth in Ehrlich's ascites tumor hosting mice
[2]
.
Oxythiamine chloride hydrochloride (250 or 500 mg/kg, daily for 5 week) inhibits tumor cell metastasis via inhibition of MMPs in mice implanted (s.c.) with LLC cells
[4]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Ehrlich's ascites tumor hosting mice [2] |
| Dosage: | 100-500 mg/kg |
| Administration: | i.p., 4 days |
| Result: | Inhibited tumor growth by 43% at 300 mg/kg and 84% at 500 mg/kg. |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
DMSO : 50 mg/mL ( 147.82 mM ; ultrasonic and warming and heat to 60°C)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.9564 mL | 14.7820 mL | 29.5639 mL |
| 5 mM | 0.5913 mL | 2.9564 mL | 5.9128 mL |
| 10 mM | 0.2956 mL | 1.4782 mL | 2.9564 mL |
-
1.
References
- [1] Wang J, et al. Inhibition of transketolase by oxythiamine altered dynamics of protein signals in pancreatic cancer cells. Exp Hematol Oncol. 2013 Jul 27;2:18.
- [2] Raïs B, et al. Oxythiamine and dehydroepiandrosterone induce a G1 phase cycle arrest in Ehrlich's tumor cells through inhibition of the pentose cycle. FEBS Lett. 1999 Jul 30;456(1):113-8.
- [3] Bai L, et al. A dose- and time-dependent effect of oxythiamine on cell growth inhibition in non-small cell lung cancer. Cogn Neurodyn. 2022 Jun;16(3):633-641.
- [4] Yang CM, et al. The in vitro and in vivo anti-metastatic efficacy of oxythiamine and the possible mechanisms of action. Clin Exp Metastasis. 2010 May;27(5):341-9.
Synonyms
