[CAS NO. 62480-44-8] L-Leucine-7-amido-4-methylcoumarinhydrochloride
PRODUCTS SPECIFICATIONS [62480-44-8]
DESCRIPTION [62480-44-8]
Overview
| MDL | MFCD00043262 |
|---|---|
| Molecular Weight | 324.80 |
| Molecular Formula | C16H21ClN2O3 |
| SMILES | CC(C(C(O1)=C2)=CC=C2NC([C@@H](N)CC(C)C)=O)=CC1=O.Cl |
Summary
L-Leucine-7-amido-4-methylcoumarin (Leu-AMC) hydrochloride is a bright blue fluorogenic peptidyl substrate for LAP3 (leucine aminopeptidase). L-Leucine-7-amido-4-methylcoumarin hydrochloride can be used for leucine aminopeptidase inhibition assays in vitro [1] .
In Vitro
In leucine aminopeptidases inhibition assays in KB 3.1 cells. Compounds 3 and 5 are observed to weakly inhibit the biofilm formation by
S. aureus
and constrain inhibitory activity of ʟ-Leu-AMC hydrolysis in KB 3.1 cells
[1]
.
The amount of hydrolyzed 7-amino-4-methylcoumarin (AMC) is determined in a fluorescence spectrophotometer (excitation/emission: 365/440 nm)
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL ( 307.88 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.0788 mL | 15.3941 mL | 30.7882 mL |
| 5 mM | 0.6158 mL | 3.0788 mL | 6.1576 mL |
| 10 mM | 0.3079 mL | 1.5394 mL | 3.0788 mL |
