[CAS NO. 66893-81-0] POBN
PRODUCTS SPECIFICATIONS [66893-81-0]
DESCRIPTION [66893-81-0]
Overview
| MDL | MFCD00041022 |
|---|---|
| Molecular Weight | 194.23 |
| Molecular Formula | C10H14N2O2 |
| SMILES | [O-][N+]1=CC=C(C=C1)/C=[N+](C(C)(C)C)\[O-] |
Summary
POBN (4-POBN) is a cell permeable, hydrophilic spin trap that can be used to detect free radical adducts [1] .
In Vitro
When POBN (20 mM) and Sodium formate (100 mM) are mixed in the collecting tube with bile in the presence of 2,2′-dipyridyl (DP) (5 mM) and bathocuproinedisulfonic acid disodium salt hydrate (BC) (5 mM), only a minor residual signal of POBN radical adduct is observed [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Electron spin resonance (ESR) spectrum of radical adducts detected in bile of rats 1 h after acute Sodium formate (2 g/kg) and POBN (1.5 g/kg) i.p. administration [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Fischer male rats (300-400 g) [1] |
| Dosage: | 1.5 g/kg (Pharmacokinetic Analysis) |
| Administration: | Injected simultaneously i.p. |
| Result: | A strong six-line ESR signal of the POBN radical adduct was detected in the bile of rats after acute sodium formate poisoning. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
H 2 O : 100 mg/mL ( 514.85 mM ; Need ultrasonic)
DMSO : 50 mg/mL ( 257.43 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 5.1485 mL | 25.7427 mL | 51.4854 mL |
| 5 mM | 1.0297 mL | 5.1485 mL | 10.2971 mL |
| 10 mM | 0.5149 mL | 2.5743 mL | 5.1485 mL |
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1.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (514.85 mM); Clear solution; Need ultrasonic
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2.
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3.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (12.87 mM); Clear solution
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4.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (12.87 mM); Clear solution
Synonyms
