[CAS NO. 75168-11-5] Acridine Orange 10-Nonyl Bromide
PRODUCTS SPECIFICATIONS [75168-11-5]
DESCRIPTION [75168-11-5]
Overview
| MDL | MFCD00467605 |
|---|---|
| Molecular Weight | 472.50 |
| Molecular Formula | C26H38BrN3 |
| SMILES | CCCCCCCCC[N+]1=C2C=C(N(C)C)C=CC2=CC3=C1C=C(N(C)C)C=C3.[Br-] |
1 Publications Citing Use of MCE
Summary
Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λ ex : 489 nm, λ em : 525 nm).
In Vivo
Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λ ex : 489 nm, λ em : 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria [1] . when Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 33.33 mg/mL ( 70.54 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.1164 mL | 10.5820 mL | 21.1640 mL |
| 5 mM | 0.4233 mL | 2.1164 mL | 4.2328 mL |
| 10 mM | 0.2116 mL | 1.0582 mL | 2.1164 mL |
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Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1.67 mg/mL (3.53 mM); Clear solution
References
- [1] Ratinaud MH, et al. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry. 1988 May;9(3):206-12.
- [2] Gallet PF, et al. Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange. Eur J Biochem. 1995 Feb 15;228(1):113-9.
