[CAS NO. 901-47-3] TAME

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PRODUCTS SPECIFICATIONS [901-47-3]

Catalog

HY-13255

Brand

MCE

CAS

901-47-3

DESCRIPTION [901-47-3]

Overview

MDL	MFCD01320524
Molecular Weight	342.41
Molecular Formula	C14H22N4O4S
SMILES	CC1=CC=C(C=C1)S(N[C@H](C(OC)=O)CCCNC(N)=N)(=O)=O

For research use only. We do not sell to patients.

Summary

TAME is an inhibitor of anaphase-promoting complex/cyclosome (APC/C or APC) , which binds to APC/C and prevents its activation by Cdc20 and Cdh1, produces mitotic arrest. TAME is not cell permeable ^[1] ^[2] .

IC50 & Target

Anaphase-promoting complex (APC) ^[1]

In Vitro

The absence of APC substrates, TAME ejects Cdc20 from the APC by promoting Cdc20 auto-ubiquitination in its N-terminal region. Cyclin B1 antagonizes TAME's effect by promoting binding of free Cdc20 to the APC and suppressing Cdc20 auto-ubiquitination ^[2] .
TAME stabilizes cyclin B1 in Xenopus extract by two mechanisms. First, it reduces the k _cat of the APC ^Cdc20 /cyclin B1 complex without affecting the K _m , slowing the initial ubiquitination of unmodified cyclin B1. Second, as cyclin B1 becomes ubiquitinated, it loses its ability to promote Cdc20 binding to the APC in the presence of TAME. As a result, cyclin B1 ubiquitination terminates before reaching the threshold necessary for proteolysis ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL ( 292.05 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9205 mL	14.6024 mL	29.2048 mL
5 mM	0.5841 mL	2.9205 mL	5.8410 mL
10 mM	0.2920 mL	1.4602 mL	2.9205 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

L-Arginine, N²-[(4-methylphenyl)sulfonyl]-, methyl ester

Arginine, N²-(p-tolylsulfonyl)-, methyl ester, L-

Arginine, (p-tolylsulfonyl)-, methyl ester

TAME (pharmaceutical)

α-N-p-Toluenesulfonyl-L-arginine methyl ester

Methyl p-toluenesulfonyl-L-argininate

Methyl N^α-p-toluenesulfonyl-L-argininate

α-Tosyl-L-arginine methyl ester

p-Toluenesulfonyl-L-arginine methyl ester

p-Tolylsulfonyl-L-arginine methyl ester

N¹-Tosyl-L-arginine methyl ester

p-Tosyl-L-arginine methyl ester

N-α-Toluenesulfonyl-L-arginine methyl ester

α-N-p-Tosyl-L-arginine methyl ester

TAME

(S)-Methyl 5-guanidino-2-(4-methylphenylsulfonamido)pentanoate