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[CAS NO. 901-47-3] TAME
| Ships within | Stock | Price | Qty | Total |
Please click "REQUEST A QUOTE" button if there is no stock, or you need other sizes or custom synthesis.
| Catalog: | HY-13255 |
| Brand: | MCE |
| CAS: | 901-47-3 |
Overview
| MDL | MFCD01320524 |
|---|---|
| Molecular Weight | 342.41 |
| Molecular Formula | C14H22N4O4S |
| SMILES | CC1=CC=C(C=C1)S(N[C@H](C(OC)=O)CCCNC(N)=N)(=O)=O |
Summary
IC50 & Target
Anaphase-promoting complex (APC) [1]
In Vitro
The absence of APC substrates, TAME ejects Cdc20 from the APC by promoting Cdc20 auto-ubiquitination in its N-terminal region. Cyclin B1 antagonizes TAME's effect by promoting binding of free Cdc20 to the APC and suppressing Cdc20 auto-ubiquitination
[2]
.
TAME stabilizes cyclin B1 in
Xenopus
extract by two mechanisms. First, it reduces the k
cat
of the APC
Cdc20
/cyclin B1 complex without affecting the K
m
, slowing the initial ubiquitination of unmodified cyclin B1. Second, as cyclin B1 becomes ubiquitinated, it loses its ability to promote Cdc20 binding to the APC in the presence of TAME. As a result, cyclin B1 ubiquitination terminates before reaching the threshold necessary for proteolysis
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 100 mg/mL ( 292.05 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.9205 mL | 14.6024 mL | 29.2048 mL |
| 5 mM | 0.5841 mL | 2.9205 mL | 5.8410 mL |
| 10 mM | 0.2920 mL | 1.4602 mL | 2.9205 mL |
References
- [1] Zeng X, et al. Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. Cancer Cell. 2010 Oct 19;18(4):382-95.
- [2] Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. Nat Chem Biol. 2012 Feb 26;8(4):383-92.
Synonyms
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