[CAS NO. 3963-95-9] Methacyclinehydrochloride
PRODUCTS SPECIFICATIONS [3963-95-9]
DESCRIPTION [3963-95-9]
Overview
| MDL | MFCD04972012 |
|---|---|
| Molecular Weight | 478.88 |
| Molecular Formula | C22H23ClN2O8 |
| SMILES | O[C@@](C(O)=C(C(C1=C2C=CC=C1O)=O)[C@](C2=C)([H])[C@@H]3O)(C(C(C(N)=O)=C4O)=O)[C@]3([H])[C@@H]4N(C)C.Cl |
2 Publications Citing Use of MCE
Summary
Methacycline hydrochloride is a tetracycline antibiotic and can inhibits bacterial protein synthesis. Methacycline hydrochloride is a potent epithelial-mesenchymal transition (EMT) inhibitor. Methacycline hydrochloride blocks EMT in vitro and fibrogenesis in vivo without directly affecting TGF-β1 Smad signaling. Methacycline hydrochloride is an antimicrobial and has the potential for pulmonary fibrosis [1] .
IC50 & Target
|
Tetracycline |
In Vitro
Methacycline hydrochloride is an inhibitor of A549 EMT with an IC50 of roughly 5 μM
[1]
.
In vitro, Methacycline hydrochloride (10, 20 μM; for 48 hours) inhibits TGF-β1-induced α-smooth muscle actin, Snail1, and collagen I of primary alveolar epithelial cells. Methacycline hydrochloride inhibits TGF-β1–induced non-Smad pathways, including c-Jun N-terminal kinase, p38, and Akt activation, but not Smad or β-catenin transcriptional activity. Methacycline has no effect on baseline c-Jun N-terminal kinase, p38, or Akt activities or lung fibroblast responses to TGF-β1
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
In vivo, Methacycline hydrochloride (100 mg/kg/day; ip; beginning 10 days after intratracheal Bleomycin) improves survival at Day 17. Bleomycin-induced canonical EMT markers, Snail1, Twist1, collagen I, as well as fibronectin protein and mRNA, ARE attenuated by Methacycline hydrochloride (Day 17)
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 50 mg/mL ( 104.41 mM ; Need ultrasonic)
H 2 O : 6.67 mg/mL ( 13.93 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.0882 mL | 10.4410 mL | 20.8821 mL |
| 5 mM | 0.4176 mL | 2.0882 mL | 4.1764 mL |
| 10 mM | 0.2088 mL | 1.0441 mL | 2.0882 mL |
-
1.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (5.22 mM); Clear solution
Synonyms