[CAS NO. 1041631-93-9] Kuguaglycoside C
PRODUCTS SPECIFICATIONS [1041631-93-9]
DESCRIPTION [1041631-93-9]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 616.83 |
| Molecular Formula | C36H56O8 |
| SMILES | C=C(C)/C=C/C[C@@H](C)[C@@]1([H])CC[C@@]([C@]1(C)CC2)(C)[C@]([C@]2(C=O)[C@@](CC[C@H](O)C3(C)C)([H])C3=C4)([H])[C@H]4O[C@]5([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O |
Summary
Kuguaglycoside C is a triterpene glycoside that can be isolated from the leaves of Momordica charantia . Kuguaglycoside C induces caspase‐independent DNA cleavage and cell death of neuroblastoma cells. Kuguaglycoside C also significantly increases the expression and cleavage of apoptosis-inducing factor ( AIF ) [1] .
In Vitro
Kuguaglycoside C (0-100 μM, 48 h) induces significant cytotoxicity against the IMR-32 cells, with an IC
50
of 12.6 μM
[1]
.
Kuguaglycoside C induces nuclear shrinkage at a high concentration (100 μM) in examination by Hoechst 33342 staining, but no apoptotic bodies were observed on flow cytometry
[1]
.
Kuguaglycoside C (30 μM, 48 h) shows no activation of caspase-3 or caspase-9, but shows increase in the levels of TRADD, AIF and DFF45, and decrease in the levels of RIP1 and survivin
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | IMR-32 cells |
| Concentration: | 0, 0.3, 1, 3, 10, 30, 100 μM |
| Incubation Time: | 48 h |
| Result: | Induced significant cytotoxicity against the IMR-32 cells, with an IC 50 of 12.6 μM. |
Western Blot Analysis [1]
| Cell Line: | IMR-32 cells |
| Concentration: | 30 μM |
| Incubation Time: | 0, 2, 4, 8, 24, 48 h |
| Result: | Activated both type I (caspase-dependent) and type II (caspase-independent) DNases. Showed significant PARP cleavage. Showed increase in the levels of TRADD, AIF and DFF45, and decrease in the levels of RIP1 and survivin. Protein levels of cleaved caspase‐3, ‐7 and ‐9 were not increased. The expression levels of p‐p38 MAPK, p‐JNK, Bax, Bcl‐2 and cytochrome c remained unchanged. |
Cell Cycle Analysis [1]
| Cell Line: | IMR-32 cells |
| Concentration: | 1, 3, 10, 30, 100 μM |
| Incubation Time: | 48 h |
| Result: | Had no effect on the cells of any phase of the cell cycle in the neuroblastoma cell line. |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.