[CAS NO. 1596362-29-6] PS372424 hydrochloride

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PRODUCTS SPECIFICATIONS [1596362-29-6]

Catalog

AS967222

Brand

Arctom Scientific

CAS

1596362-29-6

DESCRIPTION [1596362-29-6]

Overview

MDL	-
Molecular Weight	625.20
Molecular Formula	C33H45ClN6O4
SMILES	O=C([C@H]1N(C(CCC(C2=CC=CC=C2)=O)=O)CC3=C(C=CC=C3)C1)N[C@H](C(NCC4CCCCC4)=O)CCCNC(N)=N.[H]Cl

For research use only. We do not sell to patients.

Summary

PS372424 hydrochloride, a three amino-acid fragment of CXCL10, is a specific human CXCR3 agonist with anti-inflammatory activity. PS372424 hydrochloride prevents human T-cell migration in a humanized model of arthritic inflammation ^[1] ^[2] .

IC50 & Target

Human CXCR3

In Vitro

PS372424 (100 ng/mL) increases p-Erk1 and p-Erk2 in U87-CXCR3-A cells after 5 min of stimulation ^[1] .
PS372424 (10-200 nM; 30 min) causes a concentration-dependent phosphorylation of CCR5 on CXCR3 ⁺ T cells not in CXCR3 ^- T cells ^[2] .
PS372424 competes for binding of radiolabeled CXCL10 to membranes prepared from HEK293/CXCR3 Gqi5 cells with an IC ₅₀ of 42±21 nM ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis ^[2]

Cell Line:	CXCR3 ⁺ T cells and CXCR3 ^- T cells
Concentration:	10, 50, 100, or 200 nM
Incubation Time:	30 minutes
Result:	Caused a concentration-dependent phosphorylation of CCR5 on CXCR3 ⁺ T cells. Did not result in phosphorylation of CCR5 on CXCR3 ^- T cells.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL ( 399.87 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5995 mL	7.9974 mL	15.9949 mL
5 mM	0.3199 mL	1.5995 mL	3.1990 mL
10 mM	0.1599 mL	0.7997 mL	1.5995 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution

* All of the co-solvents are available by MCE.