[CAS NO. 1875036-75-1] BI8626
PRODUCTS SPECIFICATIONS [1875036-75-1]
DESCRIPTION [1875036-75-1]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 440.54 |
| Molecular Formula | C25H28N8 |
| SMILES | NCC1=CC=CC(CNC2=C(C3=NC=N2)N=CN=C3N4CCN(CC5=CC=CC=C5)CC4)=C1 |
Summary
BI8626 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC 50 of 0.9 μM [1] .
IC50 & Target
IC50: 0.9 μM (HUWE1) [1]
In Vitro
BI8626 induces HUWE1 ectopically expresses to abolishe ubiquitination of MCL1 in HeLa cells
[1]
.
BI8626 suppresses colony formation of Ls174T cells with estimated IC
50
value of 0.7 μM, and BI8622 (1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1
[1]
.
BI8626 (0-50 μM; 0-6 hours) retards the degradation of MCL1 in response to UV irradiation to the same extent as depletion of HUWE1 in U2OS cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Cycle Analysis [1]
| Cell Line: | Ls174T cells |
| Concentration: | 0 μM, 5 μM,10 μM, 15 μM, 20 μM |
| Incubation Time: | 0-4 days |
| Result: | Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. |
Western Blot Analysis [1]
| Cell Line: | U2OS cells |
| Concentration: | 0 μM, 20 μM, 50 μM |
| Incubation Time: | 0 hour,1 hour,2 hours,4 hours,6 hours |
| Result: | Retarded the degradation of MCL1 in response to UV irradiation in HeLa cells by inhibiting HUWE1 in U2OS cells. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 83.33 mg/mL ( 189.15 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.2699 mL | 11.3497 mL | 22.6994 mL |
| 5 mM | 0.4540 mL | 2.2699 mL | 4.5399 mL |
| 10 mM | 0.2270 mL | 1.1350 mL | 2.2699 mL |
-
1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.08 mg/mL (4.72 mM); Clear solution