[CAS NO. 2242470-43-3] DCZ0415
PRODUCTS SPECIFICATIONS [2242470-43-3]
DESCRIPTION [2242470-43-3]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 356.42 |
| Molecular Formula | C23H20N2O2 |
| SMILES | O=C1N(C2=CC=C(CC3=CC=NC=C3)C=C2)C(C4C5C(C6)C6C(C=C5)C14)=O |
1 Publications Citing Use of MCE
Summary
IC50 & Target
|
NF-κB |
In Vitro
DCZ0415 (10, 20 μM; 72 hours) shows a significant decrease in colony formation, indicating it inhibits cell proliferation
[1]
.
DCZ0415 (1.25-40 μM; 72 hours) induces a significant dose-dependent decrease of viability in
MM cells
[1]
.
DCZ0415 (10, 20 μM; 24-72 hours) shows a dose-dependent relationship between DCZ0415 treatment and apoptotic cell death
[1]
.
DCZ0415 (10, 20 μM; 24 hours) induces a significant accumulation in G0/G1 MM cells
[1]
.
DCZ0415 (10 μM; 48 hours) decreases the protein levels of phosphorylated (p)-iκBα and phosphorylated (p)-NF-κB in MM cells
[1]
.
DCZ0415 has IC
50
s of 1.0–10 μM in CalcuSyn in MM cell lines
[1]
.
DCZ0415 exerts cytotoxic effects by inhibiting DNA 288 synthesis in MM cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | Multiple myeloma (MM) cells |
| Concentration: | 10, 20 μM |
| Incubation Time: | 72 hours |
| Result: | Showed a significant decrease in colony formation, indicating it inhibits cell proliferation. |
Cell Viability Assay [1]
| Cell Line: | MM cells |
| Concentration: | 1.25, 2.5, 5, 10, 20, 40 μM |
| Incubation Time: | 72 hours |
| Result: | Induced a significant dose-dependent decrease of viability. |
Apoptosis Analysis [1]
| Cell Line: | MM cells |
| Concentration: | 10, 20 μM |
| Incubation Time: | 24, 48, 72 hours |
| Result: | Showed a dose-dependent relationship between DCZ0415 treatment and apoptotic cell death. |
Cell Cycle Analysis [1]
| Cell Line: | MM cells |
| Concentration: | 10 and 20 μM |
| Incubation Time: | 24 hours |
| Result: | Induced a significant accumulation in G0/G1 MM cells. |
Western Blot Analysis [1]
| Cell Line: | MM cells |
| Concentration: | 10 μM |
| Incubation Time: | 48 hours |
| Result: | Decreased the protein levels of phosphorylated (p)-iκBα and phosphorylated (p)-NF-κB in MM cells. |
In Vivo
DCZ0415 (ip; 50 mg/kg/day for 14 days) significantly reduces the growth of MM cells-induced tumors in immune-deficient mice [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Nude mice (6-weeks-old) with H929 775 cells [1] |
| Dosage: | 50 mg/kg |
| Administration: | Intraperitoneal injection; every day for 14 days |
| Result: | Significantly reduced the growth of MM cells-induced tumors. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 62.5 mg/mL ( 175.35 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.8057 mL | 14.0284 mL | 28.0568 mL |
| 5 mM | 0.5611 mL | 2.8057 mL | 5.6114 mL |
| 10 mM | 0.2806 mL | 1.4028 mL | 2.8057 mL |