[CAS NO. 332108-65-3] ABMA
PRODUCTS SPECIFICATIONS [332108-65-3]
DESCRIPTION [332108-65-3]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 350.29 |
| Molecular Formula | C18H24BrNO |
| SMILES | COC1=CC=C(Br)C=C1CNC2(C3)CC4CC3CC(C4)C2 |
Summary
ABMA is a broad-spectrum inhibitor of intracellular toxins and pathogens. ABMA efficiently protects cells against various toxins and pathogens including viruses , intracellular bacteria and parasite . ABMA selectively acts at host cell late endosomes rather than targeting toxin or pathogen itself. ABMA has broad-spectrum anti-infection activity [1] [2] .
In Vitro
ABMA protects cells against four bacterial toxins (
Corynebacterium diphtheriae
(DT; EC
50
of 62.9 μM),
Bacillus anthracis
(LT),
Clostridium difficile
toxin B (TcdB; EC
50
of 73.3 µM),
Clostridium sordellii
lethal toxin (TcsL; EC
50
of 86.7 μM)), three viruses (Ebola (EC
50
of 3.3 µM), rabies (EC
50
of 19.4 µM), dengue-4 virus ( EC
50
of 8.2 µM)), two species of Chlamydiales intracellular bacteria (
Simkania negevensis
and
Chlamydia trachomatis
), and the parasite
Leishmania infantum
(EC
50
of 7.1 µM) at micromolar level
[1]
.
In A549 cells, ABMA treatment induces a decrease in ricin cytotoxicity with an EC
50
of 3.8 µM, and a protection factor (R) at 30 µM ranging from 5 to 10. ABMA retained almost 100% of its biological activity against ricin-induced cytotoxicity up to six days
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
ABMA (2-200 mg/kg; intraperitoneal injection; female BALB/c mice) treatment protects mice from nasal instillation of an LD 90 of ricin [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Pathogen-free female BALB/c mice (6 week-old) with ricin [1] |
| Dosage: | 2 mg/kg, 20 mg/kg, 200 mg/kg |
| Administration: | Intraperitoneal injection |
| Result: | A statistically significant protection according to survival curves was observed with a single ip dose of 2 mg/kg. The 20 mg/kg dose fully protected animals through to day 21. The 200 mg/kg dose resulted in 80% of protection of mice against ricin challenge with a single animal succumbing on day 15. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : 125 mg/mL ( 356.85 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.8548 mL | 14.2739 mL | 28.5478 mL |
| 5 mM | 0.5710 mL | 2.8548 mL | 5.7096 mL |
| 10 mM | 0.2855 mL | 1.4274 mL | 2.8548 mL |
References
- [1] Wu Y, et al. ABMA, a small molecule that inhibits intracellular toxins and pathogens by interfering with late endosomal compartments.
- [2] Wu Y, et al. DABMA: A Derivative of ABMA with Improved Broad-Spectrum Inhibitory Activity of Toxins and Viruses. ACS Med Chem Lett. 2019 Jul 2;10(8):1140-1147.