[CAS NO. 39023-73-9] NHC-diphosphate
PRODUCTS SPECIFICATIONS [39023-73-9]
DESCRIPTION [39023-73-9]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 419.18 |
| Molecular Formula | C9H15N3O12P2 |
| SMILES | O[C@H]([C@@H]1O)[C@@](N(C=C/C2=N\O)C(N2)=O)([H])O[C@@H]1CO[P](O[P](O)(O)=O)(O)=O |
1 Publications Citing Use of MCE
Summary
NHC-diphosphate is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a diphosphate form [1] . NHC is a pyrimidine ribonucleoside and behaves as a potent anti-virus agent . NHC effectively inhibits the replication of venezuelan equine encephalitis virus (VEEV), Chikungunya virus (CHIKV) and hepatitis C virus (HCV) [1] .
In Vitro
Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC.
Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP; HY-135867D) can be observed, while NHC-triphosphate remains the most abundant metabolite.
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
H 2 O : 160 mg/mL ( 381.70 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.3856 mL | 11.9281 mL | 23.8561 mL |
| 5 mM | 0.4771 mL | 2.3856 mL | 4.7712 mL |
| 10 mM | 0.2386 mL | 1.1928 mL | 2.3856 mL |
References
- [1] Brenda I Hernandez-Santiago, et al. Metabolism of the anti-hepatitis C virus nucleoside beta-D-N4-hydroxycytidine in different liver cells. Antimicrob Agents Chemother. 2004 Dec;48(12):4636-42
- [2] Maryam Ehteshami, et al. Characterization of β-d- N4-Hydroxycytidine as a Novel Inhibitor of Chikungunya Virus.
