[CAS NO. 471-95-4] Bufotalin
PRODUCTS SPECIFICATIONS [471-95-4]
DESCRIPTION [471-95-4]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 444.56 |
| Molecular Formula | C26H36O6 |
| SMILES | C[C@@]1(CC[C@@]2([H])[C@@]3([H])CC[C@@]4([H])[C@@]2(CC[C@H](O)C4)C)[C@@H](C(C=C5)=COC5=O)[C@@H](OC(C)=O)C[C@@]13O |
3 Publications Citing Use of MCE
Summary
In Vitro
Bufotalin (0.1-2.5 μM; 12-96 hours) treatment dose- and time-dependently inhibits MG-63 osteoblastoma cell survival
[1]
.
Bufotalin (0.5-2.5 μM; 48 hours) treatment dose-dependently increases the percentage of Annexin V positive cells (apoptotic cells) and caspase-12 activity in MG-63 cells. Bufotalin-induced osteoblastoma cell apoptosis is associated with caspase-12 activation
[1]
.
Bufotalin (0.5-2.5 μM; 12 hours) treatment dose-dependently induces C/EBP homologous protein (CHOP) expression as well as PERK and IRE1 phosphorylation in MG-63 cells. Bufotalin induces endoplasmic reticulum (ER) stress activation in cells
[1]
.
Bufotalin treatment induces cell cycle arrest at G2/M phase through down-regulation of Aurora A, CDC25, CDK1, cyclin A and cyclin B1, as well as up-regulation of p53 and p21 in HepG2 cells. Bufotalin treatment also induces apoptosis which was accompanied by decrease in mitochondrial membrane potential, increases in intracellular calcium level and reactive oxygen species production, activations of caspase-9 and -3, cleavage of poly ADP-ribose polymerase (PARP) as well as changes in the expressions of bcl-2 and bax
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | MG-63 osteoblastoma cells |
| Concentration: | 0.1 μM, 0.5 μM, 1 μM, 2.5 μM |
| Incubation Time: | 12 hours, 24 hours, 48 hours, 72 hours, 96 hours |
| Result: | Inhibited MG-63 osteoblastoma cell survival. |
Apoptosis Analysis [1]
| Cell Line: | MG-63 cells |
| Concentration: | 0.5 μM, 1 μM, 2.5 μM |
| Incubation Time: | 48 hours |
| Result: | Dose-dependently increased the percentage of Annexin V positive cells (apoptotic cells) and caspase-12 activity in MG-63 cells. |
Western Blot Analysis [1]
| Cell Line: | MG-63 cells |
| Concentration: | 0.5 μM, 1 μM, 2.5 μM |
| Incubation Time: | 12 hours |
| Result: | Dose-dependently induced CHOP expression as well as PERK and IRE1 phosphorylation in MG-63 cells. |
In Vivo
Bufotalin (0.5-1 mg/kg; intraperitoneal injection; twice daily; for 7 days) treatment shows a significantly reduced tumor growth in mice [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | SCID male mice (4-6 weeks old) injected with U2OS cells [1] |
| Dosage: | 0.5 mg/kg, 1 mg/kg |
| Administration: | Intraperitoneal injection; twice daily; for 7 days |
| Result: | Inhibited U2OS osteoblastoma cell growth in mice. |
Appearance
Solid
Source
toad
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
Solvent & Solubility
DMSO : ≥ 4.6 mg/mL ( 10.35 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.2494 mL | 11.2471 mL | 22.4942 mL |
| 5 mM | 0.4499 mL | 2.2494 mL | 4.4988 mL |
| 10 mM | 0.2249 mL | 1.1247 mL | 2.2494 mL |
References
- [1] Zhu YR, et al. Bufotalin-induced apoptosis in osteoblastoma cells is associated with endoplasmic reticulum stress activation. Biochem Biophys Res Commun. 2014 Aug 15;451(1):112-8.
- [2] Zhang DM, et al. Bufotalin from Venenum Bufonis inhibits growth of multidrug resistant HepG2 cells through G2/M cell cycle arrest and apoptosis. Eur J Pharmacol. 2012 Oct 5;692(1-3):19-28.
Synonyms