[CAS NO. 480-54-6] Retrorsine
PRODUCTS SPECIFICATIONS [480-54-6]
DESCRIPTION [480-54-6]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 351.39 |
| Molecular Formula | C18H25NO6 |
| SMILES | O=C(O[C@]1([H])CCN2[C@]1([H])C(COC([C@](CO)(O)[C@H](C)C/3)=O)=CC2)C3=C/C |
Summary
In Vitro
Retrorsine (60-240 μM; 24 hours) significantly reduces HSEC-CYP3A4 cells viability and GSH levels, and increases formation of pyrrole-protein adducts
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [3]
| Cell Line: | HSEC-CYP3A4 cells |
| Concentration: | 60 μM, 120 μM , 240 μM |
| Incubation Time: | 24 hours |
| Result: | Significantly decreased cell viability. |
In Vivo
Retrorsine (30 mg/kg; i.p.; twice) impairs liver regeneration in the PBL model not only by an S or G2/M phase block, but also by a block located before the G1/S transition of the cell cycle
[4]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Male Wistar rats (180±20 g), portal branch ligation (PBL) model [4] |
| Dosage: | 30 mg/kg |
| Administration: | Intraperitoneal injection, twice, separated by 2-week interval |
| Result: | Strongly impaired the liver weight gain, protein and DNA synthesis as well as induction of cell cycle related proteins in the regenerating lobes after PBL. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL ( 284.58 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.8458 mL | 14.2292 mL | 28.4584 mL |
| 5 mM | 0.5692 mL | 2.8458 mL | 5.6917 mL |
| 10 mM | 0.2846 mL | 1.4229 mL | 2.8458 mL |
References
- [1] F J Cubero, et al. Hepatic proliferation in Gunn rats transplanted with hepatocytes: effect of retrorsine and tri-iodothyronine. Cell Prolif. 2005 Jun;38(3):137-46.
- [2] S Laconi, et al. Liver regeneration in response to partial hepatectomy in rats treated with retrorsine: a kinetic study. J Hepatol. 1999 Dec;31(6):1069-74.
- [3] Yao Lu, et al. Establishment of a novel CYP3A4-transduced human hepatic sinusoidal endothelial cell model and its application in screening hepatotoxicity of pyrrolizidine alkaloids. J Environ Sci Health C Toxicol Carcinog. 2020;38(2):169-185.
- [4] Christian Picard, et al. Retrorsine: a kinetic study of its influence on rat liver regeneration in the portal branch ligation model. J Hepatol. 2003 Jul;39(1):99-105.
Synonyms