[CAS NO. 56563-17-8] PluriSIn #2
PRODUCTS SPECIFICATIONS [56563-17-8]
DESCRIPTION [56563-17-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 249.20 |
| Molecular Formula | C11H8FN3O3 |
| SMILES | O=C(N(C(N1)=O)C=C(F)C1=O)NC2=CC=CC=C2 |
1 Publications Citing Use of MCE
Summary
PluriSIn #2 is a selective transcriptional inhibitor of topoisomerase II α (TOP2A) . PluriSIn #2 is a compound that selectively eliminates undifferentiated human pluripotent stem cells (hPSCs) [1] .
IC50 & Target
|
topoisomerase II alpha |
In Vitro
PluriSIn #2 (20 µM; 12 hours) treatment reduces TOP2A expression at the protein level
[1]
.
A 24h exposure of PluriSIn #2 (20 µM) is sufficient to induce massive cell death in hPSCs, but doesn’t affect the viability of various other cell types derived from them
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | B153 and HUES9 human pluripotent stem cells (hPSCs) |
| Concentration: | 20 µM |
| Incubation Time: | 12 hours |
| Result: | Reduced TOP2A expression at the protein level. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 5 mg/mL ( 20.06 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.0128 mL | 20.0642 mL | 40.1284 mL |
| 5 mM | 0.8026 mL | 4.0128 mL | 8.0257 mL |
| 10 mM | 0.4013 mL | 2.0064 mL | 4.0128 mL |
