[CAS NO. 7488-99-5] α-Carotene
PRODUCTS SPECIFICATIONS [7488-99-5]
DESCRIPTION [7488-99-5]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 536.87 |
| Molecular Formula | C40H56 |
| SMILES | CC1(C)[C@@H](/C=C/C(C)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C2=C(C)CCCC2(C)C)C(C)=CCC1 |
Summary
In Vitro
α-Carotene (0.5-2.5 μM; 24 hours) significantly increases protein expression of TIMP-1 and TIMP-2 in a concentration-dependent manner in LLC cells. AC (0.5-2.5 μM) significantly increases PAI-1 protein expression. α-Carotene (2.5 μM) also significantly inhibits integrin β1-mediated phosphorylation of focal adhesion kinase (FAK) which then decreased the phosphorylation of MAPK family
[2]
.
α-Carotene (0.5, 1, 2.5 μM; 48 hours) significantly and concentration-dependently inhibits invasion of LLC during 48 h of incubation
[2]
.
α-Carotene (0.5, 1, 2.5 μM; 24 hours) significantly decreases activities of MMP-9, -2 and uPA in concentration-dependent manner in LLC cells
[2]
.
α-Carotene (2, 5, 10 μM; 7 days) inhibits the proliferation of the human neuroblastoma cell line GOTO in a dose- and time-dependent manner. α-Carotene (5 μM; 48 hours) halts the cell cycle at the G0/G1 phase concomitantly with a reduction in the mRNA expression of the protooncogene N-Myc
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [2]
| Cell Line: | Lewis lung carcinoma (LLC) cells |
| Concentration: | 0.5, 1, 2.5 μM |
| Incubation Time: | 24 hours |
| Result: | Significantly increased protein expression of TIMP-1 and TIMP-2 in a concentration-dependent manner in LLC cells. |
In Vivo
α-Carotene (5 mg/kg; oral; twice a week; for additional 3 weeks) alone significantly decreases lung metastasis without affecting primary tumor growth
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | C57BL/6 male mice (4 weeks old; 20-25 g) with LLC cells [2] |
| Dosage: | 5 mg/kg |
| Administration: | Oral; twice a week; for additional 3 weeks |
| Result: | Significantly decreased lung metastasis. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
References
- [1] Bushway, R.J., et al. Determination of α- and β-carotene in fruit and vegetables by high performance liquid chromatography. Can. Inst. Food Sci. Technol. J. 15(3), 165-169 (1982).
- [2] Liu YZ, et al. Alpha-carotene inhibits metastasis in Lewis lung carcinoma in vitro, and suppresses lungmetastasis and tumor growth in combination with taxol in tumor xenografted C57BL/6 mice. J Nutr Biochem. 2015 Jun;26(6):607-15.
- [3] Murakoshi M, et al. Inhibitory effects of alpha-carotene on proliferation of the human neuroblastoma cell line GOTO. J Natl Cancer Inst. 1989 Nov 1;81(21):1649-52.
Synonyms