[CAS NO. ] AT-1002TFA

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PRODUCTS SPECIFICATIONS

Catalog

HY-114426A

Brand

MCE

CAS

-

DESCRIPTION

Overview

MDL	-
Molecular Weight	821.91
Molecular Formula	C34H54F3N9O9S
SMILES	N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CS)C(N[C@@H]([C@H](CC)C)C(NCC(N[C@@H](CCCNC(N)=N)C(N[C@@H](CC(C)C)C(O)=O)=O)=O)=O)=O)=O.OC(C(F)(F)F)=O

For research use only. We do not sell to patients.

Summary

AT-1002 TFA, a 6-mer synthetic peptide ^[1] , is a tight junction regulator and absorption enhancer ^[2] .

In Vitro

AT-1002, a 6-mer synthetic peptide, belongs to an emerging novel class of compounds that reversibly increase paracellular transport of molecules across the epithelial barrier. AT-1002 can undergo Cys-Cys dimerization ^[1] .
Undifferentiated Caco-2 cells are treated with AT-1002 (0 to 5 mg/mL, 3 or 24 hours) and viability is assessed by measuring cellular ATP content. Treatment with AT-1002 for up to 3 h does not affect cell viability at any concentration. In particular, the viability of Caco-2 cells is not affected by 5 mg/mL AT-1002. AT-1002 reduces cell viability after 24 h at concentrations of 2.5 mg/mL and higher. However, the cells remain viable after 24 h if the cells are washed after exposure to AT-1002 for 3 h indicating that AT-1002 does not irreversibly damage cells ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay ^[2]

Cell Line:	Caco-2 cells
Concentration:	0 to 5 mg/mL
Incubation Time:	3 or 24 hours
Result:	Treatment for up to 3 h did not affect cell viability at any concentration. Reduced cell viability after 24 h at concentrations of 2.5 mg/mL and higher.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL ( 40.55 mM ; Need ultrasonic)

H ₂ O : 1 mg/mL ( 1.22 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2167 mL	6.0834 mL	12.1668 mL
5 mM	0.2433 mL	1.2167 mL	2.4334 mL
10 mM	0.1217 mL	0.6083 mL	1.2167 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: PBS

Solubility: 8.33 mg/mL (10.13 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
2.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.5 mg/mL (3.04 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (3.04 mM); Clear solution
4.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (3.04 mM); Clear solution

* All of the co-solvents are available by MCE.