[CAS NO. 2714087-26-8] JH295hydrate
PRODUCTS SPECIFICATIONS [2714087-26-8]
DESCRIPTION [2714087-26-8]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 338.36 |
| Molecular Formula | C18H18N4O3 |
| SMILES | C#CC(NC1=CC2=C(NC(/C2=C\C3=C(C)N=C(CC)N3)=O)C=C1)=O.[H]O[H] |
Summary
JH295 hydrate is a potent, irreversible and selective NIMA-related kinase 2 (Nek2) inhibitor with an IC 50 of 770 nM. JH295 hydrate inhibits cellular Nek2 via alkylation of Cys22. JH295 hydrate is inactive against the mitotic kinases, Cdk1 , Aurora B or Plk1 , and does not perturb bipolar spindle assembly or the spindle assembly checkpoint [1] .
IC50 & Target
IC50: 770 nM (Nek2) [1]
In Vitro
JH295 (Compound 16; 0.08-20 μM; 45 minutes; RPMI7951 cells) treatment inhibits WT Nek2 in cells with an IC 50 of ~1.3 μM, whereas it has little effect on the C22V mutant [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis [1]
| Cell Line: | RPMI7951 cells |
| Concentration: | 0.08 μM, 0.25 μM, 0.74 μM, 2.2 μM, 6.6 μM, 20 μM |
| Incubation Time: | 45 minutes |
| Result: | Inhibited WT Nek2 in cells with an IC50 of ~1.3 μM, whereas had little effect on the C22V mutant. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
| Powder | -20°C | 3 years |
|---|---|---|
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
