[CAS NO. 2989537-78-0] QTX125TFA
PRODUCTS SPECIFICATIONS [2989537-78-0]
DESCRIPTION [2989537-78-0]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 531.44 |
| Molecular Formula | C25H20F3N3O7 |
| SMILES | O=C(C1=C(C2=COC=C2)C=C(C3=CC=C(O)C=C3)N1)NCC4=CC=C(C(NO)=O)C=C4.O=C(O)C(F)(F)F |
Summary
IC50 & Target
|
HDAC6 |
In Vitro
QTX125 (25-500 nM; 24-48 hours) TFA treatment induces the subsequent apoptosis demonstrated by annexin V/propidium iodide double staining and the cleavage of caspase-9, caspase-8, caspase-3, and PARP
[1]
.
In MCL cell lines MINO, REC-1, IRM-2 and HBL-2 cells, QTX125 TFA (10 nM, 10 μM, 100 μM) induces dose-dependent hyperacetylation of α-tubulin
[1]
.
QTX125 TFA has the strongest growth-inhibitory effect in Burkitt cell lymphoma, follicular lymphoma, and mantle cell lymphoma (MCL)
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Apoptosis Analysis [1]
| Cell Line: | MINO, REC-1, IRM-2 and HBL-2 cells |
| Concentration: | 25 nM, 50 nM, 100 nM, 500 nM |
| Incubation Time: | 24 hours, 48 hours |
| Result: | Inhibited annexin V/propidium iodide double staining. |
Western Blot Analysis [1]
| Cell Line: | MINO, REC-1, IRM-2 and HBL-2 cells |
| Concentration: | 25 nM, 50 nM, 100 nM, 500 nM |
| Incubation Time: | 24 hours |
| Result: | Inhibited the cleavage of caspase-9, caspase-8, caspase-3, and PARP. |
In Vivo
QTX125 TFA (60 mg/kg; i.p.; daily dosing for 5 days; for 4 weeks) treatment inhibits tumor growth in REC-1 or MINO cells xenografted in nude mice [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Nude mice bearing REC-1 or MINO cells [1] |
| Dosage: | 60 mg/kg |
| Administration: | Intraperitoneal administration; daily dosing for 5 days; for 4 weeks |
| Result: | Inhibited tumor growth in REC-1 or MINO cells xenografted in nude mice. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 125 mg/mL ( 235.21 mM ; Need ultrasonic)
H 2 O : < 0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.8817 mL | 9.4084 mL | 18.8168 mL |
| 5 mM | 0.3763 mL | 1.8817 mL | 3.7634 mL |
| 10 mM | 0.1882 mL | 0.9408 mL | 1.8817 mL |
-
1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (3.91 mM); Clear solution
