[CAS NO. ] NP213TFA

Name: NP213TFA
Brand: Arctom Scientific
SKU: HY-126810A

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PRODUCTS SPECIFICATIONS

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Catalog

HY-126810A

Brand

MCE

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-

DESCRIPTION

Overview

MDL	-
Molecular Weight	1207.32
Molecular Formula	C44H85F3N28O9
SMILES	-

For research use only. We do not sell to patients.

Summary

NP213 TFA is a rapidly acting, novel, first-in-class synthetic antimicrobial peptide (AMP) , has anti-fungal activities. NP213 TFA targets the fungal cytoplasmic membrane and plays it role via membrane perturbation and disruption. NP213 TFA is effective and well-tolerated in resolving nail fungal infections ^[1] ^[2] .

IC50 & Target

IC50: fungal ^[1]

In Vitro

NP213 (500-1000 μg/mL; 18 hours) increases the number of PI-stained T. rubrum NCPF0118 cells in samples. This results suggests that NP213 is fungicidal and the mechanisms of action involved membrane permeabilization ^[1] .
NP213 is against T. rubrum NCPF0118, shows different MIC values against T. rubrum NCPF0118, and the MICs varies depending on the source of the keratin. The MIC values are 16-32 mg/L, 125 mg/L, and 250 mg/L for NP213 in 1640 media containing human nail Keratin, human skin Keratin and Lamb’s wool Keratin, respectively ^[1] .NP213 TFA (2-3 hours; 0-8 μg/ml) has great activity against clinically relevant yeast, including candida spp , Cryptococcus spp and Trichosporon spp . For all 122 yeast isolates, with the median MIC ₁₀₀ values of 1-2 µg/ml ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay ^[3]

Cell Line:	Clinically relevant yeast isolates
Concentration:	0 μg/ml; 0.125 μg/ml; 0.25 μg/ml; 0.5 μg/ml; 1 μg/ml; 2 μg/ml; 4 μg/ml; 8 μg/ml
Incubation Time:	2 h, 2.5 h, 4 h
Result:	Decreased isolates growth as a dose-dependent manner.

In Vivo

NP213 TFA (25 mg/kg) is well tolerated in mice. In Murine models of acute disseminated candidiasis, NP213 is tolerated and efficacious and exhibits a half-life of approximately 4.5 h ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility

In Vitro:

H ₂ O : 100 mg/mL ( 82.83 mM ; Need ultrasonic)

DMSO : 100 mg/mL ( 82.83 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.8283 mL	4.1414 mL	8.2828 mL
5 mM	0.1657 mL	0.8283 mL	1.6566 mL
10 mM	0.0828 mL	0.4141 mL	0.8283 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: PBS

Solubility: 100 mg/mL (82.83 mM); Clear solution; Need ultrasonic
2.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution
4.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution

* All of the co-solvents are available by MCE.