Cyclic ADP-ribose ammonium
PRODUCTS SPECIFICATIONS
DESCRIPTION
Overview
| MDL | - |
|---|---|
| Molecular Weight | - |
| Molecular Formula | C15H21N5O13P2.xNH3 |
| SMILES | O[C@H]([C@@H]1O)[C@](O[C@]1([H])COP(O)(OP(O)(OC2)=O)=O)([H])N3C(N=CN([C@]4([H])O[C@@]2([H])[C@@H](O)[C@H]4O)C5=N)=C5N=C3.N.[F,Cl,Br,I] |
Summary
Cyclic ADP-ribose ammonium (cADPR ammonium) is a potent second messenger for calcium mobilization that is synthesized from NAD + by an ADP-ribosyl cyclase. Cyclic ADP-ribose ammonium increases cytosolic calcium mainly by Ryanodine receptor -mediated release from endoplasmic reticulum and also by extracellular influx through the opening of TRPM2 channels [1] [2] [3] .
In Vitro
cADPR (20 nM) elicits a large rapid Ca
2+
release in sea urchin eggs homogenates
[1]
.
cADPR (100 µM; 10 min) induces a sustained elevation of intracellular calcium concentration in a subset (64%) of cultured astrocytes
[4]
.
cADPR (100 µM) and heat (35-38.5 ℃) stimulates oxytocin OT release from the isolated hypothalami of male mice in culture
[5]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
cADPR (100 µM; push-pull type of brain microperfusion) elevats OT concentrations in ordinate or subordinate mice [5] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
References
- [1] Galione A, et, al. Ca(2+)-induced Ca2+ release in sea urchin egg homogenates: modulation by cyclic ADP-ribose. Science. 1991 Sep 6;253(5024):1143-6.
- [2] Lee HC, et, al. Structural determination of a cyclic metabolite of NAD+ with intracellular Ca2+-mobilizing activity. J Biol Chem. 1989 Jan 25;264(3):1608-15.
- [3] Ribeiro JM, et, al. Specific cyclic ADP-ribose phosphohydrolase obtained by mutagenic engineering of Mn 2+-dependent ADP-ribose/CDP-alcohol diphosphatase. Sci Rep. 2018 Jan 18;8(1):1036.
- [4] Verderio C, et, al. Evidence of a role for cyclic ADP-ribose in calcium signalling and neurotransmitter release in cultured astrocytes. J Neurochem. 2001 Aug;78(3):646-57.
- [5] Zhong J, et, al. Cyclic ADP-Ribose and Heat Regulate Oxytocin Release via CD38 and TRPM2 in the Hypothalamus during Social or Psychological Stress in Mice. Front Neurosci. 2016 Jul 22;10:304.
