[CAS NO. ] Gap 26 TFA

Ships within

Stock

Price

Qty

Total

$0.00

Please click "REQUEST A QUOTE" button if you need other sizes or custom synthesis

request a quote

If there is no stock, or you need other sizes or custom synthesis, please:

PRODUCTS SPECIFICATIONS

Catalog

HY-P1082A

Brand

MCE

CAS

-

DESCRIPTION

Overview

MDL	-
Molecular Weight	1664.80
Molecular Formula	C72H108F3N19O21S
SMILES	-

For research use only. We do not sell to patients.

5 Publications Citing Use of MCE

Summary

Gap 26 TFA is a connexin mimetic peptide, composed of residue numbers 63-75 of the first extracellular loop of connexin 43 (gap junction blocker), containing the SHVR amino acid motif ^[1] .

In Vitro

Gap 26 (0.25 mg/mL, 30 min) reduces the wave size in the three cell lines (RBE4, SV-ARBEC and ECV304). Gap 26 (0.25 mg/mL, 30 min) completely abolishes the InsP3-triggered ATP response and reduced the ATP release to below the control level, indicating that the basal ATP release is also affected ^[1] .
Gap 26 does indeed significantly inhibit our InsP3-triggered intercellular calcium waves, but it did not have any effect on dye coupling through junctional channels as evidenced by the FRAP experiments, despite the fact that connexin 43 was present in the cell lines used ^[1] .
Gap 26 (100-300 μM) dose-dependently reduces the rhythmic responses of rabbit superior mesenteric arteries, with IC ₅₀ of 28.4 ± 3.4 μM ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL ( 60.07 mM ; Need ultrasonic)

H ₂ O : 50 mg/mL ( 30.03 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.6007 mL	3.0034 mL	6.0067 mL
5 mM	0.1201 mL	0.6007 mL	1.2013 mL
10 mM	0.0601 mL	0.3003 mL	0.6007 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: PBS

Solubility: 100 mg/mL (60.07 mM); Clear solution; Need ultrasonic
2.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.5 mg/mL (1.50 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (1.50 mM); Clear solution
4.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (1.50 mM); Clear solution

* All of the co-solvents are available by MCE.