[CAS NO. ] Gap19 TFA

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PRODUCTS SPECIFICATIONS

Catalog

HY-P1136A

Brand

MCE

CAS

-

DESCRIPTION

Overview

MDL	-
Molecular Weight	1275.46
Molecular Formula	C57H97F3N14O15
SMILES	-

For research use only. We do not sell to patients.

1 Publications Citing Use of MCE

[1]Research Square Print. 2022 Aug.

Summary

Gap19 TFA, a peptide derived from nine amino acids of the Cx43 cytoplasmic loop (CL), is a potent and selective connexin 43 (Cx43) hemichannel blocker. Gap19 TFA inhibits hemichannels caused by preventing intramolecular interactions of the C-terminus (CT) with the CL. Gap19 TFA is not blocking GJ channels or Cx40/pannexin-1 hemichannels. Gap19 TFA has protective effects against myocardial ^[1] ^[2] .

IC50 & Target

Cx43 Hemichannel ^[1]

In Vitro

Gap19 TFA (250 μM; for 30 min ) decreases mitochondrial potassium uptake ^[1] .
Gap19 TFA (400 μM) inhibits unitary hemichannel currents in HeLa-Cx43 cells ^[2] .
Gap19 TFA (100 μM) inhibits hemichannel unitary currents in ventricular cardiomyocytes ^[2] .
Gap19 TFA (250 μM, 30 min) protects against myocardial ischemia/reperfusion injury in vitro and in vivo ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Gap19 TFA (iv; 25 mg/kg; 10 min before ligation) significantly reduces the infarct size by approximately one-fifth ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57/BL6 mice ^[2]
Dosage:	25 mg/kg
Administration:	IV; 10 min before ligation
Result:	Significantly reduced the infarct size by approximately one-fifth.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL ( 78.40 mM ; Need ultrasonic)

H ₂ O : 100 mg/mL ( 78.40 mM ; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7840 mL	3.9202 mL	7.8403 mL
5 mM	0.1568 mL	0.7840 mL	1.5681 mL
10 mM	0.0784 mL	0.3920 mL	0.7840 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: PBS

Solubility: 100 mg/mL (78.40 mM); Clear solution; Need ultrasonic

* All of the co-solvents are available by MCE.