For research use only.
Storage
3 years,-20°C,powder
1 years,-80°C,in solvent
Description
RO-3306 is an ATP-competitive, and selective inhibitor with of 20 nM, >15-fold selectivity against a diverse panel of human kinases. RO-3306 enhances p53-mediated activation and .
In vitro
RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.
