[CAS NO. 494753-69-4] Ginsenoside Rk1
PRODUCTS SPECIFICATIONS [494753-69-4]
DESCRIPTION [494753-69-4]
Overview
| MDL | MFCD28124355 |
|---|---|
| Molecular Weight | 767.00 |
| Molecular Formula | C42H70O12 |
| SMILES | C[C@]12[C@@](C[C@@H](O)[C@@]3([H])[C@]2(CC[C@@H]3C(CC/C=C(C)/C)=C)C)([H])[C@@]4([C@@](C(C)([C@@H](O[C@@]5([H])[C@@H]([C@H]([C@H](O)[C@@H](CO)O5)O)O[C@]6([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]6O)CO)CC4)C)([H])CC1)C |
1 Publications Citing Use of MCE
Summary
Ginsenoside Rk1 is a unique component created by processing the ginseng plant (mainly Sung Ginseng, SG) at high temperatures [1] . Ginsenoside Rk1 has anti-inflammatory effect, suppresses the activation of Jak2/Stat3 signaling pathway and NF-κB [2] . Ginsenoside Rk1 has anti-tumor effect, antiplatelet aggregation activities, anti-insulin resistance, nephroprotective effect, antimicrobial effect, cognitive function enhancement, lipid accumulation reduction and prevents osteoporosis [1] . Ginsenoside Rk1 induces cell apoptosis by triggering intracellular reactive oxygen species ( ROS ) generation and blocking PI3K/Akt pathway [3] .
In Vitro
Ginsenoside Rk1 (0-40 μM; 6 hours) inhibits MCP-1 and TNF-α mRNA induced by lipopolysaccharide (LPS), expression of IL-1β is inhibited at 40 μM
[2]
.
Ginsenoside Rk1 (0-40 μM; 24 hours) inhibits phosphorylation of JAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner
[2]
.
Ginsenoside Rk1 (0-160 μM; 48 hours) results in cell viability significant decrease 75.52 ± 2.51% (40 μM), 52.72 ± 2.54% (80 μM), 17.41 ± 2.94% (120 μM)and 12.63 ± 3.24% (160 μM) compared with control
[3]
.
Ginsenoside Rk1 (0-120 μM; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/M phase proportion decrease in MDA-MB-231 cells
[3]
.
Ginsenoside Rk1 (0-120 μM; 24 hours) promotes the percentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleus fragmentation, condensation and apoptotic body formation
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
RT-PCR [2]
| Cell Line: | RAW264.7 cells |
| Concentration: | 10 μM, 20 μM, 40 μM |
| Incubation Time: | 6 hours |
| Result: | Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells. |
Western Blot Analysis [2]
| Cell Line: | RAW264.7 cells |
| Concentration: | 10 μM, 20 μM, 40 μM |
| Incubation Time: | 6 hours |
| Result: | Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells |
Cell Viability Assay [3]
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 48 hours |
| Result: | Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner. |
Cell Cycle Analysis [3]
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 24 hours |
| Result: | Induced G0/G1 phase arrest. |
Apoptosis Analysis [3]
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 24 hours |
| Result: | Induced apoptosis in MDA-MB-231 cells. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL ( 130.38 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.3038 mL | 6.5189 mL | 13.0378 mL |
| 5 mM | 0.2608 mL | 1.3038 mL | 2.6076 mL |
| 10 mM | 0.1304 mL | 0.6519 mL | 1.3038 mL |
References
- [1] Elshafay A, et al. Ginsenoside Rk1 bioactivity: a systematic review. PeerJ. 2017 Nov 17;5:e3993.
- [2] Yu Q,et al. Ginsenoside Rk1 suppresses pro-inflammatory responses in lipopolysaccharide-stimulated RAW264.7 cells by inhibiting the Jak2/Stat3 pathway. Chin J Nat Med. 2017 Oct;15(10):751-757.
- [3] Hong Y, et al. Ginsenoside Rk1 induces cell cycle arrest and apoptosis in MDA-MB-231 triple negative breast cancer cells. Toxicology. 2019 Apr 15;418:22-31.
