[CAS NO. 108524-94-3] Ilexgenin A
PRODUCTS SPECIFICATIONS [108524-94-3]
DESCRIPTION [108524-94-3]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 502.68 |
| Molecular Formula | C30H46O6 |
| SMILES | C[C@@]12C([C@@]3([H])[C@@](CC2)(CC[C@H]([C@]3(O)C)C)C(O)=O)=CC[C@@]4([H])[C@]1(CC[C@]5([H])[C@@]4(CC[C@@H]([C@]5(C)C(O)=O)O)C)C |
Summary
Ilexgenin A is a pentacyclic triterpenoid, which extracted from Ilex hainanensis Merr. Ilexgenin A can be used for the research of inflammation and cancer [1] .
In Vitro
Ilexgenin A (0~100 μM; 24 hours; HT 29 and HCT 116 cells) effectively decreases cell viability in HT 29 and HCT 116 cells
[1]
.
Ilexgenin A (12.5~50 μM; 24 hours; HT 29 cells) downregulates the expression of SREBP-1, inhibits the translocation of SREBP-1 to the nucleus and decreases the content of TG
[1]
.
Ilexgenin A (12.5~50 μM; HT 29 and HCT 116 cells) induces cell cycle arrest at G1 phase in colon cancer cells
[1]
.
Ilexgenin A (12.5~50 μM; 24 hours; HCT 116 cells) modulates lipid metabolism. Ilexgenin A (50 μM; 24 hours; HT 29 and HCT 116 cells) regulates SREBP-1 by inhibiting HIF1α
[1]
.
Ilexgenin A inhibits fatty acids synthesis
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | HT 29 and HCT 116 cells |
| Concentration: | 0~100 μM |
| Incubation Time: | 24 hours |
| Result: | Effectively decreased cell viability in HT 29 and HCT 116 cells. |
Western Blot Analysis [1]
| Cell Line: | HT 29 cells |
| Concentration: | 12.5~50 μM |
| Incubation Time: | 24 hours |
| Result: | Downregulated the expression of SREBP-1. |
RT-PCR [1]
| Cell Line: | HT 29 cells |
| Concentration: | 12.5~50 μM |
| Incubation Time: | 24 hours |
| Result: | Decreased the content of TG. |
Immunofluorescence [1]
| Cell Line: | HT 29 cells |
| Concentration: | 12.5~50 μM |
| Incubation Time: | 24 hours |
| Result: | Inhibited the translocation of SREBP-1 to the nucleus. |
In Vivo
Ilexgenin A (20 mg/kg) ameliorates the AOM/DSS induced carcinogenesis. Ilexgenin A can regulate lipid metabolism. Ilexgenin A decreases the expression of SREBP-1 and HIF 1α in AOM/DSS mice [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL ( 198.93 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.9893 mL | 9.9467 mL | 19.8934 mL |
| 5 mM | 0.3979 mL | 1.9893 mL | 3.9787 mL |
| 10 mM | 0.1989 mL | 0.9947 mL | 1.9893 mL |
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1.
-
2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (4.97 mM); Clear solution
Synonyms