[CAS NO. 1169755-45-6] INDY

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PRODUCTS SPECIFICATIONS [1169755-45-6]

Catalog

HY-108476

Brand

MCE

CAS

1169755-45-6

DESCRIPTION [1169755-45-6]

Overview

MDL	MFCD28100814
Molecular Weight	235.30
Molecular Formula	C12H13NO2S
SMILES	CC(/C=C1SC2=CC=C(O)C=C2N\1CC)=O

For research use only. We do not sell to patients.

2 Publications Citing Use of MCE

Summary

INDY is a potent and ATP-competitive Dyrk1A and Dyrk1B inhibitor with IC ₅₀ s of 0.24 μM and 0.23 μM, respectively. INDY binds in the ATP pocket of the enzyme and has a K _i value of 0.18 μM for Dyrk1A. INDY sharply reduces the self-renewal capacity of normal and tumorigenic cells in primary Glioblastoma (GBM) cell lines and neural progenitor cells ^[1] ^[2] .

IC50 & Target

IC50: 0.24 μM (Dyrk1A) and 0.23 μM (Dyrk1B) ^[1]
Ki: 0.18 μM (Dyrk1A) ^[1]

In Vitro

INDY (0.3-30 μM; 20 hours) mildly inhibits tau-phosphorylation at 3 μM, and nearly completely inhibits at 30 μM ^[1] .
INDY effectively reverses the aberrant tau-phosphorylation and rescues the repressed NFAT (nuclear factor of activated T cell) signalling induced by Dyrk1A (dual-specificity tyrosine-(Y)-phosphorylation-regulated kinase 1A) overexpression ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis ^[1]

Cell Line:	COS7 cells transfected with either EGFP-Dyrk1A and EGFP-tau
Concentration:	0.3, 1, 3, 10, 30 μM
Incubation Time:	20 hours
Result:	Mildly inhibited tau-phosphorylation at 3 μM, and nearly completely inhibited at 30 μM.

In Vivo

ProINDY (2.5 μM) recoveres apparently normal development of the Xenopus embryo ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 33.33 mg/mL ( 141.65 mM )

* "≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2499 mL	21.2495 mL	42.4989 mL
5 mM	0.8500 mL	4.2499 mL	8.4998 mL
10 mM	0.4250 mL	2.1249 mL	4.2499 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (10.62 mM); Clear solution

* All of the co-solvents are available by MCE.